Thermo Fisher Scientific
Quantitative proteomics strategies using Tandem Mass Tags (TMT) enable precise measurement of protein abundance from multiple samples into a single high-resolution LC-MS analysis. Increasingly, various biological experiments demand higher multiplexing and sample throughput with accurate quantitation. In this webinar, our expert speakers will lay out strategies that combine next generation technologies in multiplexing chemistry, hardware, and software to achieve increased quantitative performance and throughput efficiency on Thermo Scientific Orbitrap Eclipse Tribrid mass spectrometer. This includes approaches utilizing next-generation isobaric tags, Real-Time Search for SPS MS3, and gas-phase fractionation with the Thermo Scientific FAIMS Pro interface. Together, these advances allow for intelligent acquisition methods that improve quantitation accuracy, precision, and proteome depth.
Devin Karl Schweppe, Ph.D., Research Fellow in Cell Biology, Harvard Medical School
Dr. Devin Schweppe works at Harvard Medical School with Dr. Steven Gygi to develop methods and resources for multiplexed quantitative proteomics workflows. Specifically, Dr. Schweppe has been focused on real-time acquisition management protocols to improve instrument efficiency and quantitative accuracy.
Aaron Robitaille, Ph.D., Senior Product Marketing Manager, Thermo Fisher Scientific
Aaron Robitaille has focused his career on using mass spectrometry to understand how eukaryotic cells dynamically and quantitatively respond to their environment to ensure proper cell growth and determine cell fate. He received his Ph.D. from the University of Basel in Switzerland. There, as a member of Michael Hall’s laboratory, Aaron utilized phosphoproteomics to identify a mechanism by which the conserved kinase mTOR regulated pyrimidine synthesis and promoted cell proliferation. He then completed his post-doctoral research at the University of Washington in Seattle, WA in Randall Moon laboratory. Aaron used multiple quantitative proteomic approaches to measure changes in protein expression and protein-protein interactions to identity regulators of embryonic stem cell differentiation. These works were significant to understanding the fundamental biology of how cells coordinate the regulation of signaling networks and metabolism to properly make cell fate decisions, such as whether to proliferate or arrest, self-renew or differentiated. Currently, Aaron Robitaille is a Senior Product Applications Specialist for Thermo Fisher Scientific in San Jose, CA. He is focused on supporting quantitative proteomics workflows that use Tandem Mass Tags (TMTs), and continues to help develop innovative mass spectrometry technology to answer challenging biological questions.
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