Understanding when to apply comparative or absolute quantitation in real-time PCR is essential for obtaining data that is useful and appropriate for your application. Absolute quantitation determines actual copy numbers but is labor-intensive. Relative quantitation is less arduous but provides only relative abundance of target sequences. This handbook explains the critical tradeoffs of precision vs. difficulty for these two approaches. It lays out what researchers can expect from typical workflows for both methods, including the role of standard curves in each. Eliminate the mystery of quantitation decisions by downloading this handbook today.
In addition to comparative vs. quantitation tips, the “Real-time PCR handbook” is the definitive source for all things related to real-time PCR, including:
- Basics of real-time PCR
- Experimental design
- Data analysis
- Digital PCR
Download your FREE copy of the “Real-time PCR handbook” today!