NPM1 Antibody (MA325200A488) in IF

Immunofluorescent analysis of Na/K ATPase Alpha (green) in A431 cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product ## 37525) for 30 minutes at room temperature. Cells were stained with a Na/K ATPase Alpha Monoclonal Antibody, AlexaFluor 488 conjugate (Product ## MA3928A488) at a dilution of 5ug/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product ## 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

Immunofluorescent analysis of Na/K ATPase Alpha (green) in A431 cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product ## 37525) for 30 minutes at room temperature. Cells were stained with a Na/K ATPase Alpha Monoclonal Antibody, AlexaFluor 488 conjugate (Product ## MA3928A488) at a dilution of 5ug/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product ## 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

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