Performance of Top 12 Abundant Protein Depletion Spin Columns compared to equivalent products from other suppliers
Human serum (10-20μL, Part No. 31876) was loaded onto each resin and processed according to the manufacturer’s protocol (Agilent: Human 14 Multiple Affinity Removal Spin Cartridge, Part No. 51886560; Sigma: SEPPRO* IgY14 spin column, Part No. SEP010-1Kit; BAC: CaptureSelect* HumanPlasma 14 Depletion Resin, Part No. 991410) protocol. Total protein in the depleted fractions was estimated using BCA protein Assay (Thermo Scientific, Part No. 23225). Total amount of albumin in the depleted fractions was determined using AssayMax* Human Albumin ELISA Kit (Assaypro, Part No. EA2201-1). FT = flow-through (i.e., depleted sample); E = eluate (i.e., proteins that were bound by the resin, plus stripped affinity antibodies of the column). Performance of all for products is comparable in this analysis. With the Top 12 proteins removed, low-abundance proteins are now visible in each depleted sample lane (FT).