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Invitrogen
Cytokeratin 8 Monoclonal Antibody (LP3K), eBioscience™
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 10
Cytokeratin 8 Antibody (14-9938-82) in ICC/IF
Immunofluorescence analysis of Cytokeratin 8 was performed using 70% confluent log phase Caco-2 cells. The cells were fixed with ice-cold acetone at 4°C for 5 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Cytokeratin 8 Monoclonal Antibody (LP3K), eBioscience™ (Product # 14-9938-82) at a concentration of 1 µg/mL in 0.1% BSA, incubated at 4 degree celsius ove... View More
Product Details
14-9938-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
LP3K
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10557242
Product Specific Information
Description: This LP3K monoclonal antibody reacts with human cytokeratin 8 (K8), a 55-kDa member of the family of intermediate filament proteins. Cytokeratin 8 is a type II (or basic) keratin that is expressed in epithelial and carcinoma cells. Cytokeratins form the intracellular cytoskeletal network that maintains the integrity and stability of cells and tissues. More specifically, studies have demonstrated the involvement of cytokeratin 8 in protection against apoptosis, stress, or injury, as well as regulation of the cell cycle. This keratin is frequently co-expressed with cytokeratin 18, a type I (or acidic) keratin as a heterodimer. Although detected primarily in the cytoplasm of normal healthy cells, cytokeratin 8 has been found to localize to the plasma membrane in some tumor cells. Finally, cytokeratin 8 is phosphorylated on serine 73 in dividing cells.
Applications Reported: This LP3K antibody has been reported for use in immunoblotting (WB) and immunocytochemistry.
Applications Tested: This LP3K antibody has been tested by western blot analysis of reduced A549 cell lysate, as well as by immunocytochemistry of A549 cells. For western blotting, this antibody can be used at 1-10 µg/mL. For immunocytochemistry, LP3K can be used at 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. Cutokeratin 8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CK-8; Cytokeratin-8; K8; keratin 8, type II; Keratin, type II cytoskeletal 8; Keratin-8; Type-II keratin Kb8
Gene Aliases: CARD2; CK-8; CK8; CYK8; K2C8; K8; KO; KRT8
UniProt ID: (Human) P05787
Entrez Gene ID: (Human) 3856
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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