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IL-5 Antibody (500-P55BT-1MG) in WB
Western Blot: To detect Murine IL-5 by Western Blot analysis IL-5 Polyclonal Antibody, Biotin (Product # 500-P55BT-1MG) can be used at a concentration of 0.1-0.2 mg/mL. When used in conjunction with compatible development reagents, the detection limit for Recombinant Murine IL-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P55BT-1MG
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine IL-5
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930626
Product Specific Information
AA Sequence of recombinant protein: MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-5. Anti-Murine IL-5-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Murine IL-5 by sandwich ELISA (using 100 mL/well) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti- Murine IL-5 (500-P55) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-5.
Western Blot: To detect Murine IL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 mg/mL. When used in conjunction with compatible development reagents, the detection limit for Recombinant Murine IL-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P55BT-1MG will be provided as 2 x 500 µg
Target Information
IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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