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Flow Cytometry using A2B5 Monoclonal Antibody, Mouse (Catalog Number 433110) Mouse GRIP (glial-restricted immortalized precursor) cells stained with A2B5 (Blue shad) or not primary antibody (Red shade) followed by RPE-anti-IgM antibody. Samples analyzed on a BD FACS calibur cytometer and gated on cells using forward and side scatter.
|Tested species reactivity||Dog , Rabbit , Human , Mouse , Rat , Chicken , Primate|
|Host / Isotype||Mouse / IgM|
|Immunogen||Chicken embryo retina cells.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A2B5 binds to neural tissue such as retina, brain, spinal cord, and dorsal root ganglia. It is also able to bind plasma membranes of human, rat, chicken and mouse islet cells and can be used in cell sorting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.