Invitrogen

ACBD3 Monoclonal Antibody (OTI5F9)

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ACBD3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human endometrium tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a ACBD3 monoclonal antibody (Product # MA5-25999).

ACBD3 Antibody in Western Blot (WB) — Western blot analysis of ACBD3 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with ACBD3 (Product # MA5-25999) monoclonal antibody.

Product Details

MA5-25999

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:150	-
Flow Cytometry (Flow)	1:100	-

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	OTI5F9
Immunogen	Full length human recombinant protein of ACBD3 produced in HEK293T cell View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2723827

Target Information

GOLPH1, also known as GCP60, was initially identified as a Golgi protein that can interact with the integral membrane protein giantin and is thought to be involved in the maintenance of the Golgi structure. GOLPH1 has also been shown to interact with other Golgi proteins such as Golgin-160, a Golgi protein that can be cleaved by caspases-2, -3, and -7, leading to the nuclear localization of Golgin-160. GOLPH1 interaction with the Golgin-160 fragments is stronger than that with the intact Golgin-160, with its interaction regulated by the oxidation state of Cys-463 within GOLPH1, suggesting that the nuclear localization of the caspase-cleaved Golgin-160 fragments is a highly coordinated event. GOLPH1 has also been found to interact with Numb, a cytosolic signaling protein that mediates asymetric cell division of neural progenitor cells to a daughter progenitor cell and a neuron, suggesting that Golgi fragmentation and reconstitution during the cell cycle differentially regulate Numb signaling through changes in GOLPH1 subcellular distribution and may couple cell fate with cell cycle progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cells

Mytych J,Sołek P,Będzińska A,Rusinek K,Warzybok A,Tabęcka-Łonczyńska A,Koziorowski M

MA5-25999 was used in Western Blotting to provide evidence of klotho involvement in the crosstalk on the line ER-Golgi, which may, in turn, affect activation of SASP.

Tue Jan 21 00:00:00 EST 2020

Bioinformatics

Protein Aliases: Acyl-CoA-binding domain-containing protein 3; acyl-Coenzyme A binding domain containing 3; GOCAP1; golgi complex associated protein 1, 60kDa; Golgi complex-associated protein 1; Golgi phosphoprotein 1; Golgi resident protein GCP60; Golgi resident protein GCP60, N-terminally processed; GOLPH1; PBR- and PKA-associated protein 7; Peripheral benzodiazepine receptor-associated protein PAP7; PKA (RIalpha)-associated protein

Gene Aliases: ACBD3; GCP60; GOCAP1; GOLPH1; PAP7

UniProt ID: (Human) B2RB29

Entrez Gene ID: (Human) 64746

Function(s)

steroid biosynthetic process transport

Process(es)

fatty-acyl-CoA binding protein binding protein kinase A regulatory subunit binding