Immunofluorescent analysis of AICDA showing staining in the cytoplasm of Raji cells. Raji cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using an AICDA polyclonal antibody (Product # PA5-34918) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment contains a sequence corresponding to a region within amino acids 67 and 228 of AICDA|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34918 targets AICDA in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-34918 immunogen is recombinant protein fragment contains a sequence corresponding to a region within amino acids 67 and 228 of AICDA.
This gene encodes a RNA-editing deaminase that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene are the cause of autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2).
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