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Immunohistochemistry analysis of ALK / CD246 showing staining in the cytoplasm and membrane of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a ALK / CD246 Mouse Monoclonal Antibody (MA511460) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant protein corresponding to a region which spans the tyrosine kinase catalytic domain and part of the C-terminus of the NPM-ALK transcript (419-520aa)|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 3 publications below|
MA5-11460 targets ALK (Anaplastic Lymphoma Kinase) / p80 in IHC (P) applications and shows reactivity with Human samples.
The MA5-11460 immunogen is recombinant protein corresponding to a region which spans the tyrosine kinase catalytic domain and part of the C-terminus of the NPM-ALK transcript (419-520aa).
ALK/p80 is a hybrid of the anaplastic lymphoma kinase (ALK) gene and the nucleophosmin (NPM) gene resulting from the t(2;5)(p23;q35) translocation found in a third of large cell lymphomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Epstein-Barr virus-associated adrenal smooth muscle tumors and disseminated diffuse large B-cell lymphoma in a child with common variable immunodeficiency: a case report and review of the literature.
MA5-11460 was used in immunohistochemistry to investigate the clinical and pathological features in a girl with common variable immunodeficiency
|Petrilli G,Lorenzi L,Paracchini R,Ubiali A,Schumacher RF,Cabassa P,Facchetti F||International journal of surgical pathology (22:712)||2014|
Mutation-independent anaplastic lymphoma kinase overexpression in poor prognosis neuroblastoma patients.
MA5-11460 was used in immunohistochemistry to examine the therapeutic significance of anaplastic lymphoma kinase in neuroblastoma
|Passoni L,Longo L,Collini P,Coluccia AM,Bozzi F,Podda M,Gregorio A,Gambini C,Garaventa A,Pistoia V,Del Grosso F,Tonini GP,Cheng M,Gambacorti-Passerini C,Anichini A,Fossati-Bellani F,Di Nicola M,Luksch R||Cancer research (69:7338)||2009|
The EML4-ALK transcript but not the fusion protein can be expressed in reactive and neoplastic lymphoid tissues.
MA5-11460 was used in immunohistochemistry to study role of the fusion transcript/protein EML4-ALK in human carcinogenesis
|Sozzi G,Martelli MP,Conte D,Modena P,Pettirossi V,Pileri SA,Falini B||Haematologica (94:1307)||2009|
anaplastic lymphoma kinase; CD246 antigen; mutant anaplastic lymphoma kinase; NBLST3; TFG/ALK
ALK; CD246; NBLST3