|Tested species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the N-terminal residues C-K(11) K G E I F E L K A E L N S D K K E K(29) of human AP-1 complex subunit beta-1.|
|Storage buffer||whole serum|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||1:1000 - 1:5000|
|Western Blot (WB)||1:100 -1:10000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-18307 detects AP-1 complex subunit beta-1 from human and rat samples.
PA1-18307 has been successfully used in immunohistochemistry (paraffin and frozen) and Western blot procedures. Antigen retrieval has been used in testing but may not be necessary.
The PA1-18307 immunogen is a synthetic peptide corresponding to the N-terminal residues C-K(11) K G E I F E L K A E L N S D K K E K(29) of human AP-1 complex subunit beta-1, conjugated to diphtheria toxin. The sequence is 100% conserved in mouse, rat and bovine.
Reconstitute with 100 ul of distilled water. Glycerol (1:1) may be added for additional stability.
AP-1 complex subunit beta-1 is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.