|Tested species reactivity||Human|
|Host / Isotype||Sheep / IgG|
|Immunogen||Synthetic peptide from human autophagy-related protein 10.|
|Purification||Ammonium sulfate precipitation|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||10-50 µg/ml|
|Western Blot (WB)||10-50 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Reconstitute with 500 ul of distilled water.
Centrifuge to remove any insoluble material.
Autophagocytosis is a starvation-induced process responsible for transport of cytoplasmic proteins to the vacuole. Plays a role in autophagy. ATG12-conjugating enzyme (E2-like enzyme), likely serves as an ATG5-recognition molecule. By interacting with MAP1LC3A, it plays a role in the conjugation of ATG12 to ATG5. Also able to directly interact either with ATG5 or ATG7.
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