Proteintech

APOE Monoclonal Antibody (1B2C9)

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APOE Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 66830-1-IG (APOE antibody) at dilution of 1:400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

APOE Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 66830-1-IG (APOE antibody) at dilution of 1:400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

APOE Antibody in Western Blot (WB) — Human plasma tissue were subjected to SDS PAGE followed by western blot with 66830-1-Ig (APOE antibody) at a range of dilutions from 1:5000 to 1:20000 incubated at room temperature for 1.5 hours.

APOE Antibody in Western Blot (WB) — Human plasma tissue were subjected to SDS PAGE followed by western blot with 66830-1-IG (APOE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

APOE Antibody in Western Blot (WB) — Human blood tissue were subjected to SDS PAGE followed by western blot with 66830-1-Ig (APOE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

APOE Antibody in Western Blot (WB) — Figure 3 LDLR binds to both opsonized and unopsonized crystals. (A) MSU crystals (lot1-3) were opsonized in different solutions (unopsonized (HBSS), human serum, murine serum, or HBSS containing 5% BSA, 1 mg/ml fibrinogen, or 1 mg/ml LDL) at 37degC for 30 min. After washing with HBSS, the particles were incubated with 5 ug/ml recombinant His-tagged protein in HBSS + 5% BSA at 4degC for 60 min (hLDLR, mLDLR, hMARCO, hCD32b). Bound proteins were stained with anti-His Tag PE and analyzed using a flow cytometer. An unpaired, two-sided t-test was used for statistical evaluation (ns = not significant, *p < 0.05, **p < 0.01 ); MFI and SEM are shown. (B) Calcium carbonate and m-CPPD crystals were pre-incubated in HBSS or HBSS-containing 1 mg/ml hLDL and then incubated with hLDLR and stained as described in (A) . An unpaired, two-sided t-test was used for statistical evaluation (ns = not significant, **p < 0.01). Mean MFI and SEM of n=3 samples is shown. (C) Indicated crystals were opsonized in human serum at 37degC for 30 min. Incubation with recombinant LDLR was done as in (A) . Protein binding was analyzed using mouse anti-His Tag plus goat anti-mouse AlexaFluor488. Fluorescence of the samples was detected by fluorescent microscopy; scale bar = 40 um. Representative of three independent experiments. (D) Indicated crystals were opsonized with human serum at 37degC for 30 min. After incubation, particles and supernatant were separated by centrifugation: the supernatant was collected,

Product Details

66830-1-IG

Applications	Tested Dilution	Publications
Western Blot (WB)	1:3000-1:20,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:200-1:800	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Not Applicable
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	1B2C9
Immunogen	APOE Fusion Protein Ag28186
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Target Information

Apo E (Apolipoprotein E) plays an important role in the metabolism of lipids in the plasma, and is also is a constituent of various plasma lipoprotein-lipid particles. The protein is synthesized in liver, brain, spleen, and the kidney, and concentrations are high in interstitial fluids, where it plays a role in distributing cholesterol from cells where cholesterol is in excess to cells which require cholesterol. Apo E also plays an important role in the formation of very low density lipoprotein and chylomicrons. The Apo E gene is located on chromosome 19 at position 19q13. 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Binding of Macrophage Receptor MARCO, LDL, and LDLR to Disease-Associated Crystalline Structures.

Frontiers in immunology

Alberts A,Klingberg A,Hoffmeister L,Wessig AK,Brand K,Pich A,Neumann K

Published figure using APOE monoclonal antibody (Product # 66830-1-IG) in Western Blot

Mon Jun 28 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: Apo-E; ApoE4; Apolipoprotein; Apolipoprotein E; apolipoprotein E3; apolipoprotein E4; Apoprotein; B2G1; BG; MGC1571

Gene Aliases: AD2; AI255918; APO-E; APOE; LDLCQ5; LPG

UniProt ID: (Human) P02649, (Mouse) P08226

Entrez Gene ID: (Human) 348, (Mouse) 11816

Molecular Function: apolipoprotein

response to reactive oxygen species retinoid metabolic process negative regulation of endothelial cell proliferation response to dietary excess triglyceride metabolic process cholesterol catabolic process cellular calcium ion homeostasis receptor-mediated endocytosis cytoskeleton organization G-protein coupled receptor signaling pathway nitric oxide mediated signal transduction synaptic transmission, cholinergic cholesterol metabolic process regulation of gene expression negative regulation of platelet activation positive regulation of cholesterol esterification positive regulation of cholesterol efflux long-chain fatty acid transport protein import virion assembly triglyceride catabolic process cGMP-mediated signaling negative regulation of blood coagulation regulation of axon extension positive regulation of cGMP biosynthetic process neuron projection regeneration regulation of Cdc42 protein signal transduction positive regulation of low-density lipoprotein particle receptor catabolic process cholesterol efflux phospholipid efflux very-low-density lipoprotein particle remodeling low-density lipoprotein particle remodeling high-density lipoprotein particle remodeling high-density lipoprotein particle assembly chylomicron remnant clearance high-density lipoprotein particle clearance very-low-density lipoprotein particle clearance lipoprotein metabolic process lipoprotein biosynthetic process lipoprotein catabolic process vasodilation cholesterol homeostasis negative regulation of MAP kinase activity negative regulation of neuron apoptotic process negative regulation of blood vessel endothelial cell migration reverse cholesterol transport positive regulation by host of viral process negative regulation of cholesterol biosynthetic process positive regulation of lipid biosynthetic process intracellular transport regulation of neuronal synaptic plasticity artery morphogenesis negative regulation of inflammatory response positive regulation of nitric-oxide synthase activity positive regulation of membrane protein ectodomain proteolysis maintenance of location in cell fatty acid homeostasis positive regulation of dendritic spine development negative regulation of canonical Wnt signaling pathway AMPA glutamate receptor clustering NMDA glutamate receptor clustering cellular oxidant detoxification regulation of beta-amyloid clearance negative regulation of neuron death positive regulation of postsynaptic membrane organization negative regulation of presynaptic membrane organization negative regulation of beta-amyloid formation positive regulation of dendritic spine maintenance positive regulation of phospholipid efflux positive regulation of lipid transport across blood brain barrier

Process(es)

beta-amyloid binding lipid transporter activity protein binding phospholipid binding heparin binding lipid binding cholesterol binding antioxidant activity cholesterol transporter activity identical protein binding protein homodimerization activity metal chelating activity tau protein binding low-density lipoprotein particle receptor binding phosphatidylcholine-sterol O-acyltransferase activator activity very-low-density lipoprotein particle receptor binding lipoprotein particle binding