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Immunofluorescent analysis of ATP synthase B1 showing staining in the mitochondria of SKNSH cells. SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min and stained using an ATP synthase B1 polyclonal antibody (Product # PA5-30236) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 214 of Human ATP synthase B1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-30236 targets ATP synthase B1 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-30236 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 214 of Human ATP synthase B1.
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ATP synthase; ATP synthase B chain; ATP synthase B chain, mitochondrial; ATP synthase proton-transporting mitochondrial F(0) complex subunit B1; ATP synthase subunit b, mitochondrial; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, isoform 1; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1; ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1; ATPase subunit b; cel; cell proliferation-inducing protein 47; H+ transporting; H+-ATP synthase subunit b; mitochondrial; mitochondrial F0 complex; PIG47; subunit b; subunit B1