Immunofluorescent analysis of Actin Muscle Specific (green) showing staining in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Actin Muscle Specific monoclonal antibody (Product # MA5-14084) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
|Tested species reactivity||Human, Mouse, Rabbit, Rat|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||SDS extract of human myocardium|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:100-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody reacts with actin from tissue extracts of uterus, ileum, aorta, diaphragm, heart and smooth muscle cells. It recognizes alpha-actin from skeletal, cardiac, and smooth muscle, and reacts with gamma actin from smooth muscle. This antibody will react with tumors from smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle tumors (rhabdomyomas and rhabdomyosarcomas).
Staining of formalin-fixed tissues requires boiling in 1mM EDTA, pH 8.0, followed by cooling at room temperature for 20 min.
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Evidence for CD34/SMA positive cells in the left main coronary artery in atherogenesis.
MA5-14084 was used in immunohistochemistry - paraffin section to study the evidence of CD34/SMA positive cells in atherogenesis in left main coronary artery
|Kruzliak P,Hare DL,Sabaka P,Delev D,Gaspar L,Rodrigo L,Zulli A||Acta histochemica (118:413)||2016|
Plexiform fibromyxoma (plexiform angiomyxoid myofibroblastic tumor) of stomach: an unusual presentation as a fistulating abscess.
MA5-14084 was used in immunohistochemistry to report on a case of plexiform fibromyxoma of the stomach
|Lee PW,Yau DT,Lau PP,Chan JK||International journal of surgical pathology (22:286)||2014|
Expression of E-cadherin in angiomyolipoma.
MA5-14084 was used in immunohistochemistry to perform a systematic study of E-cadherin expression in angiomyolipoma
|Wang Z,Gong Q,Fan Q||Human pathology (43:2348)||2012|
Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.
MA5-14084 was used in immunohistochemistry to report on a case of sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression
|Yin WH,Li J,Chan JK||Diagnostic pathology (7:null)||2012|
Fascin expression and its potential significance in gastrointestinal stromal tumors.
MA5-14084 was used in immunohistochemistry to study the expression of fascin in gastrointestinal stromal tumors and its possible prognostic value
|Ozcan A,Karslio¿lu Y,Günal A,Cerm¿k AH,Kurt B,Ongürü O||The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology (22:363)||2011|
Tumor-in-tumor of the thyroid with basaloid differentiation: a lesion with a solid cell nest neoplastic component?
MA5-14084 was used in immunohistochemistry to report on a case of tumor-in-tumor of the thyroid with basaloid differentiation
|Eloy C,Vinagre J,Cameselle-Teijeiro J,Paiva ME,Soares P,Sobrinho-Simões M||International journal of surgical pathology (19:276)||2011|
Chorangiosis: the potential role of smoking and air pollution.
MA5-14084 was used in immunohistochemistry to investigate the role of smoking and air pollution in the development of chorangiosis
|Akbulut M,Sorkun HC,Bir F,Eralp A,Duzcan E||Pathology, research and practice (205:75)||2009|
Myointimoma of the glans penis.
MA5-14084 was used in immunohistochemistry to report on a case of myointimoma of the glans penis
|Vardar E,Gunlusoy B,Arslan M,Kececi S||Pathology international (57:158)||2007|
Ectopic cervical anaplastic ependymoma.
MA5-14084 was used in immunohistochemistry to report on a case of ectopic cervical anaplastic ependymoma
|Wang Z,Huang G,Yan P,Liang R,Wang J,Yan Q,Zhang J,Cheng H,Hu P,Ma MJ||Pathology international (55:781)||2005|
Cutaneous lymphoid hyperplasia and other lymphoid infiltrates of the breast nipple: a retrospective clinicopathologic study of fifty-six patients.
MA5-14084 was used in immunohistochemistry to study the clinicopathological spectrum of lymphoproliferations and other lymphoid infiltration of the breast nipple
|Boudova L,Kazakov DV,Sima R,Vanecek T,Torlakovic E,Lamovec J,Kutzner H,Szepe P,Plank L,Bouda J,Hes O,Mukensnabl P,Michal M||The American Journal of dermatopathology (27:375)||2005|
Epstein-Barr virus-associated smooth muscle tumour: a distinctive mesenchymal tumour of immunocompromised individuals.
MA5-14084 was used in immunohistochemistry to report on a case of Epstein-Barr virus-associated smooth muscle tumour
|Cheuk W,Li PC,Chan JK||Pathology (34:245)||2002|
G-CSF promotes the proliferation of developing cardiomyocytes in vivo and in derivation from ESCs and iPSCs.
MA5-14084 was used in immunocytochemistry to investigate the expression level of G-CSFR in vivo and the role of G-CSF during cardiomyogenesis in vitro and in vivo
|Shimoji K,Yuasa S,Onizuka T,Hattori F,Tanaka T,Hara M,Ohno Y,Chen H,Egasgira T,Seki T,Yae K,Koshimizu U,Ogawa S,Fukuda K||Cell stem cell (6:227)||2010|
Microfabrication-based modulation of embryonic stem cell differentiation.
MA5-14084 was used in immunocytochemistry to study the effect of microfabricated adhesive stencils on embryonic stem cell differentiation
|Park J,Cho CH,Parashurama N,Li Y,Berthiaume F,Toner M,Tilles AW,Yarmush ML||Lab on a chip (7:1018)||2007|