Immunohistochemistry analysis of Actin Muscle Specific showing staining in the cytoplasm (endothelial cell) of paraffin-embedded human skin squamous carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Actin Muscle Specific Mouse Monoclonal Antibody (MA511874) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Bovine, Dog, Horse, Human, Mouse, Pig, Rat|
|Published species reactivity||Fish, Zebrafish, Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody reacts with alpha-smooth muscle, alpha-skeletal and alpha-cardiac (sarcomeric). This pan muscle actin antibody reacts with tumors arising from smooth muscle (leiomysarcomas) as well as skeletal muscle tumors (rhabdomyosarcomas).
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Intestinal morphology of the wild Atlantic salmon (Salmo salar).
MA5-11874 was used in immunohistochemistry to study the morphology of the intestine in healthy wild Atlantic salmon
|Løkka G,Austbø L,Falk K,Bjerkås I,Koppang EO||Journal of morphology (274:859)||2013|
Mycophenolate mofetil and daclizumab targeting T lymphocytes in bleomycin-induced experimental scleroderma.
MA5-11874 was used in immunohistochemistry to study the ability of mycophenolate mofetil and daclizumab to prevent the development of dermal fibrosis
|Ozgen M,Koca SS,Dagli AF,Gundogdu B,Ustundag B,Isik A||Clinical and experimental dermatology (37:48)||2012|
Effectiveness of etanercept in bleomycin-induced experimental scleroderma.
MA5-11874 was used in immunohistochemistry to study the effects of etanercept and thalidomide in a murine model of bleomycin-induced scleroderma
|Koca SS,Isik A,Ozercan IH,Ustundag B,Evren B,Metin K||Rheumatology (Oxford, England) (47:172)||2008|
Smooth muscle tension induces invasive remodeling of the zebrafish intestine.
MA5-11874 was used in western blot to study epithelial cell invasion of the zebrafish intestine in response to smooth muscle tension and the underlying mechanisms
|Seiler C,Davuluri G,Abrams J,Byfield FJ,Janmey PA,Pack M||PLoS biology (10:null)||2012|