Western blot analysis of Alkyl-DHAP synthase using A) 30 µg 293T whole cell lysate and B) 30 µg whole cell lysate of human AGPS-transfected 293T cells. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an Alkyl-DHAP synthase polyclonal antibody (Product # PA5-27401) at a dilution of 1:1000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 370 and 658 of Human Alkyl-DHAP synthase|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% ProClin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The PA5-27401 immunogen is recombinant fragment corresponding to a region within amino acids 370 and 658 of Human Alkyl-DHAP synthase.
This gene is a member of the FAD-binding oxidoreductase/transferase type 4 family. It encodes a protein that catalyzes the second step of ether lipid biosynthesis in which acyl-dihydroxyacetonephosphate (DHAP) is converted to alkyl-DHAP by the addition of a long chain alcohol and the removal of a long-chain acid anion. The protein is localized to the inner aspect of the peroxisomal membrane and requires FAD as a cofactor. Mutations in this gene have been associated with rhizomelic chondrodysplasia punctata, type 3 and Zellweger syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.