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Product Details
PA5-34780
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 202 of Ang-4 (Uniprot ID#Q9Y264)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2552132
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLa, Molt-4, Raji.
IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Angiopoietin-1 (Ang-1) is a secreted ligand for Tie-2, a cell surface receptor tyrosine kinase expressed in endothelial and hemopoietic cells. Ang-1 is an angiogenic factor that mediates blood vessel maturation and may be involved in endothelial development. A related protein, angiopoietin-2 (Ang-2), is a naturally occurring antagonist of Ang-1 activation of Tie-2. In adult tissue, Ang-2 expression is restricted to sites of vascular remodeling. Ang-3 and Ang-4 represent the mouse and human counterparts of the same gene locus. The structural divergence of Ang-3 and Ang-4 cause their divergent functions. Ang-3 and Ang-4 have very different distributions in their respective species, and Ang-3 appears to act as an antagonist while Ang-4 appears to function as an agonist. Ang-3 and Ang-4 share all the main structural characteristics of Ang-1 and Ang-2 and are homologous throughout the signal peptide, N-terminal region, coiled-coil segment and Fibrinogen-like domain.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AGP4; ANG-3; ANG-4; Angiopoietin-3; Angiopoietin-4; Angiopoietin3; Angiopoietin4
Gene Aliases: ANG3; ANG4; ANGPT4
UniProt ID: (Human) Q9Y264
Entrez Gene ID: (Human) 51378
angiogenesis
endothelial cell proliferation
activation of transmembrane receptor protein tyrosine kinase activity
Notch signaling pathway
positive regulation of endothelial cell migration
negative regulation of angiogenesis
negative regulation of apoptotic process
positive regulation of blood vessel endothelial cell migration
negative regulation of blood vessel endothelial cell migration
positive regulation of angiogenesis
positive regulation of peptidyl-tyrosine phosphorylation
leukocyte migration
cellular response to hypoxia
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