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Immunofluorescent analysis of A549 cells stained with an Anterior Gradient 2 polyclonal antibody (Product # PA5-13270). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with an Anterior Gradient 2 polyclonal antibody (Product # PA5-13270) (1:25, 1 hr at 37°C). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37°C). Actin filaments have been labeled with dye-conjugated phalloidin (red). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human Anterior Gradient 2 (AGR2)|
|Purification||Size-exclusion - Dialysis, Ammonium sulfate precipitation|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anterior gradient 2 (AGR2) is known as a cancer cell marker specifically up-regulated in response to depletion of serum and oxygen. AGR2 has been identified as a tumor marker in primary and secondary cancer lesions, and as a marker for detection of circulating tumor cells (CTCs). Elevated levels of AGR2 are known to increase the metastatic potential of cancer cells, but conditions leading to increased expression of AGR2 are not well understood.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
AG-2; AG2; anterior gradient homolog 2; Anterior gradient protein 2 homolog; epididymis secretory protein Li 116; hAG-2; HPC8; protein disulfide isomerase family A, member 17; secreted cement gland homolog; Secreted cement gland protein XAG-2 homolog
AG2; AGR2; GOB-4; HAG-2; HEL-S-116; PDIA17; UNQ515/PRO1030; XAG-2