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Western blot analysis of MUC-1 (CT2) was performed by loading 25ug of HeLa (left lane) and MCF7 (right lane) cell lysates, and 10ul of PageRuler Plus Prestained Protein Ladder (Product # 26619) onto a Novex® 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product # 62288), and blocked with 5% milk in TBST for 1 hour at room temperature. Small subunits of MUC-1 were detected between ~14kD to 30kD mainly in MCF7 breast cancer cell samples using a MUC-1 monoclonal antibody (Product # MA5-11202) at a concentration of 1ug/ml overnight at 4C on a rocking platform, followed by a goat anti-Armenian hamster IgG-HRP secondary antibody (Product # PA1-32045) at a dilution of 1:10,000 for at least 1 hour at room temperature. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34076).
|Tested species reactivity||Hamster|
|Host / Isotype||Goat / IgG|
|Immunogen||Armenian Hamster IgG whole molecule|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2, with 10mg/ml BSA|
|Contains||0.01% gentamicin sulfate|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-32045 detects Hamster (Armenian) IgG from hamster samples. Greatly diminished reactivity will occur against Golden Syrian hamster IgG.
PA1-32045 has been successfully used in ELISA, and Western blot applications.
The PA1-32045 immunogen is Armenian Hamster IgG whole molecule.
Store at 4°C for short term storage, for long term storage store at -20°C or below. Avoid Freeze/Thaw Cycles.
Thermo Scientific Anti-Hamster secondary antibodies are affinity-purified antibodies with well-characterized specificity for hamster immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.