Invitrogen

Aurora A Polyclonal Antibody

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

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Datasheet

Questions & Answers

Datasheet

Questions & Answers

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Aurora A Antibody in Western Blot (WB) — Western blot analysis was performed on modified whole cell extracts (1% SDS- 30 µg lysates) of HCT 116 (Lane 1), HCT 116 treated with Paclitaxe l(100nM for 18 hrs) (Lane 2), HT-29 (Lane 3), HT-29 treated with Paclitaxel (100nM, 18hrs) (Lane 4), SK-BR-3 (Lane 5) and SK-BR-3 treated with Nocodazole (100ng/mL, 16 hrs) (Lane 6). The blot was probed with Anti-Aurora A Polyclonal Antibody (Product # PA1-26351, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 48 kDa band corresponding to AuroraA were observed across the cell lines tested, with an increase in expression upon treatment.

Aurora A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Aurora an In formalin-fixed, paraffin-embedded human pancreatic tumor tissue using an Aurora A polyclonal antibody (Product # PA1-26351) at a dilution of 1:250.

Aurora A Antibody in Immunoprecipitation (IP) — Immunoprecipitation of S35 labeled recombinant Aurora A (5.0 mg) using Aurora A polyclonal antibody (Product # PA1-26351) followed by gel electrophoresis.

Aurora A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Aurora A was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Aurora A Polyclonal Antibody (Product # PA1-26351) at 5 µg/mL concentration in 0.1% BSA, incubated at 4 degree celsius overnight (Panel a: green). Mitotic chromosomes (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing mitotic cells with AuroraA expression concentrated at the spindle poles. Panel e represents the resting cells showing cytoplasmic expression of AuroraA . Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Aurora A Antibody in Relative expression — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis of AuroraA using Aurora A Polyclonal Antibody (Product # PA1-26351) shows cytoplasmic expression, concentrated at the spindle poles in mitotic MCF7 cells, and cytoplasmic staining in resting cells. {RE}

Aurora A Antibody in Cell treatment — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Western Blot analysis of AuroraA using Aurora A Polyclonal Antibody (Product # PA1-26351) shows decreased expression of protein in HCT 116, HT-29 and SKBR3 cell lines upon treatment with Paclitaxel/ Nocodazole. {TM}

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

0.4-1 µg/mL

Immunoprecipitation (IP)

2-10 µg/mg of lysate

Immunocytochemistry (ICC/IF)

5 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide, conjugated to KLH, corresponding to a portion of human Serine/Threonine Kinase 15 encoded within exon 5.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

tris citrate/tris phosphate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4° C, do not freeze

RRID

AB_779336

Product Specific Information

PA1-26351 detects Aurora A from human samples.

Target Information

Aurora A is a 46 kDa protein that plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment.Tissue specificity: Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines. Multiple transcript variants encoding the same protein have been found for this gene. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ARK-1; AURAK; Aurora 2; Aurora A Kinase; Aurora kinase A; aurora/IP; aurora/IPL1-like kinase; Aurora/IPL1-related kinase 1; AURORA2; Breast tumor-amplified kinase; EC 2.7.11.1; hARK1; IPL1-related kinase; MGC34538; OTTHUMP00000031341; OTTHUMP00000166072; protein phosphatase 1, regulatory subunit 47; RP5-1167H4.6; serine/threonine protein kinase 15; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A

Gene Aliases: AIK; AIRK1; ARK1; AURA; AURKA; AYK1; BTAK; IAK1; PPP1R47; STK15; STK6; STK7

UniProt ID: (Human) O14965

Entrez Gene ID: (Human) 6790

Molecular Function: kinase non-receptor serine/threonine protein kinase protein kinase transferase