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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to amino acids 319-332 of human Axis Inhibitor Protein 1|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||2-5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
The Axin (Axis Inhibition) protein is a ~120 kDa negative regulator of Wnt pathway signaling which is involved in regulation of embryonic axis development in vertebrates. In vertebrates, Wnt signaling occurs when the frizzled receptor transduces a signal to the Dishevelled protein, triggering glycogen synthase kinase-3 (GSK-3) inactivation and the accumulation of beta-catenin. Axin binds directly to GSK-3 beta, beta-catenin, and the adenomatous polyposis coli (APC) tumor suppressor protein at separate binding sites; these four proteins form a tetrameric complex that regulates beta-catenin stability and signaling. In addition to its role in Wnt signaling, Axin has been implicated in tumorigenesis, particularly via its interaction with APC. Axin is ubiquitously expressed in almost all tissues, from early development to adulthood. Studies of mutant Axin protein have demonstrated that the phosphorylated form of Axin is more stable than the unphosphorylated form. Other signal transduction pathways in which Axin is involved are the JNK mitogen-associated protein kinase cascade, wherein Axin functions as a scaffold for JNK activation and the TGF- beta signaling pathway, in which Axin acts as an adapter protein for SMAD3, enhancing the transcriptional activity of TGF- beta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
AXIN; axin-1; axis inhibition protein 1; axis inhibitor 1; fused; fused, mouse, homolog of; homolog of; LA16c-314G4.3; mouse; PPP1R49; protein phosphatase 1; protein phosphatase 1, regulatory subunit 49; regulatory subunit 49
AXIN; AXIN1; PPP1R49