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|Tested species reactivity||Human|
|Published species reactivity||Fruit fly, Primate, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the internal region of the human Axin protein. Based on amino acid sequence, this antibody is not expected to cross-react with Axin-2.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
AXIN1 is a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain and is thought to function as a negative regulator of the WNT signaling pathway that regulates embryonic axis formation. AXIN1 interacts with adenomatosis polyposis coli (APC), beta-catenin, glycogen synthase kinase 3 beta, forming a tetrameric complex resulting in the regulation of the stabilization of beta-catenin. Mutations in the AXIN1 gene have been associated various carcinomas, indicating that it also functions as a tumor suppressor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Wnt signalling pathway parameters for mammalian cells.
34-5900 was used in western blot to predict Wnt signaling in human colorectal cancers.
|Tan CW,Gardiner BS,Hirokawa Y,Layton MJ,Smith DW,Burgess AW||PloS one (7:null)||2012|
Dissecting molecular differences between Wnt coreceptors LRP5 and LRP6.
34-5900 was used in western blot to elucidate the contributions of LRP5 and LRP6 to Wnt signaling.
|MacDonald BT,Semenov MV,Huang H,He X||PloS one (6:null)||2011|
|Nuclear-cytoplasmic shuttling of Axin regulates subcellular localization of beta-catenin.||Cong F,Varmus H||Proceedings of the National Academy of Sciences of the United States of America (101:2882)||2004|
|Human||Not Cited||Frequent loss of the AXIN1 locus but absence of AXIN1 gene mutations in adenocarcinomas of the gastro-oesophageal junction with nuclear beta-catenin expression.||Koppert LB,van der Velden AW,van de Wetering M,Abbou M,van den Ouweland AM,Tilanus HW,Wijnhoven BP,Dinjens WN||British journal of cancer (90:892)||2004|
|Human||Not Cited||Reduced expression of Axin correlates with tumour progression of oesophageal squamous cell carcinoma.||Nakajima M,Fukuchi M,Miyazaki T,Masuda N,Kato H,Kuwano H||British journal of cancer (88:1734)||2003|
|Human||Not Cited||Epstein-Barr virus activates beta-catenin in type III latently infected B lymphocyte lines: association with deubiquitinating enzymes.||Shackelford J,Maier C,Pagano JS||Proceedings of the National Academy of Sciences of the United States of America (100:15572)||2003|
AXIN; axin-1; axis inhibition protein 1; axis inhibitor 1; fused; fused, mouse, homolog of; homolog of; LA16c-314G4.3; mouse; PPP1R49; protein phosphatase 1; protein phosphatase 1, regulatory subunit 49; regulatory subunit 49
AXIN; AXIN1; PPP1R49