|Tested species reactivity||Human|
|Published species reactivity||Fruit fly, Non-human primate, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the internal region of the human Axin protein. Based on amino acid sequence, this antibody is not expected to cross-react with Axin-2.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
AXIN1 is a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain and is thought to function as a negative regulator of the WNT signaling pathway that regulates embryonic axis formation. AXIN1 interacts with adenomatosis polyposis coli (APC), beta-catenin, glycogen synthase kinase 3 beta, forming a tetrameric complex resulting in the regulation of the stabilization of beta-catenin. Mutations in the AXIN1 gene have been associated various carcinomas, indicating that it also functions as a tumor suppressor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Wnt signalling pathway parameters for mammalian cells.
34-5900 was used in western blot to predict Wnt signaling in human colorectal cancers.
|Tan CW,Gardiner BS,Hirokawa Y,Layton MJ,Smith DW,Burgess AW||PloS one (7:null)||2012|
Dissecting molecular differences between Wnt coreceptors LRP5 and LRP6.
34-5900 was used in western blot to elucidate the contributions of LRP5 and LRP6 to Wnt signaling.
|MacDonald BT,Semenov MV,Huang H,He X||PloS one (6:null)||2011|
|Not Applicable||Not Cited||
Identification of the suppressive factors for human immunodeficiency virus type-1 replication using the siRNA mini-library directed against host cellular genes.
34-5900 was used in western blot to screen for host factors that modulate human immunodeficiency virus type-1 replication
|Kameoka M,Kitagawa Y,Utachee P,Jinnopat P,Dhepakson P,Isarangkura-na-ayuthaya P,Tokunaga K,Sato H,Komano J,Yamamoto N,Oguchi S,Natori Y,Ikuta K||Biochemical and biophysical research communications (359:729)||2007|
The adenomatous polyposis coli protein (APC) exists in two distinct soluble complexes with different functions.
34-5900 was used in western blot to identify and characterize two distinct soluble protein pools containing APC
|Penman GA,Leung L,Näthke IS||Journal of cell science (118:4741)||2005|
|Nuclear-cytoplasmic shuttling of Axin regulates subcellular localization of beta-catenin.||Cong F,Varmus H||Proceedings of the National Academy of Sciences of the United States of America (101:2882)||2004|
Differentiation of human neural progenitor cells regulated by Wnt-3a.
34-5900 was used in western blot to assess the effects of Wnt-3a and beta-catenin on the differentiation of ReNcell VM human neural progenitor cells
|Hübner R,Schmöle AC,Liedmann A,Frech MJ,Rolfs A,Luo J||Biochemical and biophysical research communications (400:358)||2010|
|Human||Not Cited||Frequent loss of the AXIN1 locus but absence of AXIN1 gene mutations in adenocarcinomas of the gastro-oesophageal junction with nuclear beta-catenin expression.||Koppert LB,van der Velden AW,van de Wetering M,Abbou M,van den Ouweland AM,Tilanus HW,Wijnhoven BP,Dinjens WN||British journal of cancer (90:892)||2004|
|Human||Not Cited||Reduced expression of Axin correlates with tumour progression of oesophageal squamous cell carcinoma.||Nakajima M,Fukuchi M,Miyazaki T,Masuda N,Kato H,Kuwano H||British journal of cancer (88:1734)||2003|
|Human||Not Cited||Epstein-Barr virus activates beta-catenin in type III latently infected B lymphocyte lines: association with deubiquitinating enzymes.||Shackelford J,Maier C,Pagano JS||Proceedings of the National Academy of Sciences of the United States of America (100:15572)||2003|