Immunofluorescent analysis of C2C12 cells using a B-RAF polyclonal antibody (Product # PA5-14926). C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with a B-RAF polyclonal antibody (Product # PA5-14926) (1:25, 1 hr at 37°C). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37°C). Actin filaments have been labeled with dye-conjugated phalloidin (red).
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 424-453 amino acids from the central region of human B-RAF|
|Purification||Ammonium sulfate precipitation, Size-exclusion - Dialysis|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10-1:50|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with chicken and mouse based on sequence homology.
BRAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neuron. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.