|Tested species reactivity||Human|
|Host / Isotype||Chicken / IgY|
|Immunogen||Synthetic peptide corresponding to residues 20-150 of human Bcl xL.|
|Purification||Antigen affinity chromatography|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-25967 detects Bcl xL from human samples.
PA1-25967 has been successfully used in Western blot procedures. The predicted molecular weight is 28 kDa.
The PA1-25967 immunogen is a synthetic peptide corresponding to residues 20-150 of human Bcl xL.
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Bcl xL is a 241 amino acid 28 kDa member of Bcl2 family of proteins and an important regulator of apoptosis. Bcl xL forms heterodimers with BAX, BAK, and Bcl2, and its overexpression in tumor cells confers resistance against chemotherapeutic drugs. Bcl xL is phosphorylated on many sites including serine 62, a critical site for Bcl xL response to microtubule-damaging drugs such as taxol and vinblastine. Phosphorylation of serine 62 is thought to be mediated by Jun N-terminal stress kinase (JNK) signaling - negatively regulates the anti-apoptotic function of Bcl xL and controls the growth of neoplastic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.