Western blot analysis of BDNF in U87-MG lysates (lane 1), and mouse kidney lysate (lane 2) using a BDNF recombinant rabbit oligoclonal antibody (Product # 710306) at a dilution of 2ug/ml. Samples were detected using chemiluminescence (ECL). Results show a band at ~28kDa.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein corresponding to amino acids 129–247 of human BDNF|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody is predicted to react with non-human primate, rat and rabbit based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
BDNF (brain derived neurotrophic factor) is a member of the nerve growth factor family, and a prevalent growth factor of the central nervous system. BDNF is important for neuronal plasticity and survival, and maintenance of specific neuronal populations like striatal neurons. It is also involved in long-term learning and memory formation. BDNF is expressed in a glycosylated 28 kDa form containing a prodomain required for trafficking. Only after secretion is the propeptide removed by plasmin present in blood serum. BDNF has distinct isoforms derived from multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Cholesterol overload induces apoptosis in SH-SY5Y human neuroblastoma cells through the up regulation of flotillin-2 in the lipid raft and the activation of BDNF/Trkb signaling.
710306 was used in western blot to characterize induction of apoptosis in SH-SY5Y human neuroblastoma cells through up regulation of flotillin-2 in the lipid raft and the activation of BDNF/Trkb signaling due to cholesterol overload
|Huang YN,Lin CI,Liao H,Liu CY,Chen YH,Chiu WC,Lin SH||Neuroscience (328:201)||2016|