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Search Thermo Fisher Scientific
Description: The monoclonal antibody 1H11 recognizes human Bid (BH3 interacting domain death agonist), a member of the Bcl-2 family. Bid is a cytoplasmic protein that upon activation via an apoptosis signal is cleaved by caspase-8. Upon cleavage, the active Bid translocates to the mitochondria and binds BAK thereby inducing cytochrome c release and increasing mitochondrial membrane permeability. The 1H11 antibody recognizes the full length protein (24kDa).
Applications Reported: This 1H11 antibody has been reported for use in immunoblotting (WB).
Applications Tested: This 1H11 antibody has been tested by western blot analysis of human cells lines. This can be used at 1-5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
BID is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. BID is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. BID is also a pro-apoptotic member of the Bcl 2 family. BID usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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