Immunofluorescent analysis of BRCA1 in U2OS cells (nuclear foci induced by ionizing radiation). IR-treated (2 hr/4 gray IR) U2OS cells were pre-extracted with CSK buffer on ice for 4 min before fixation with 4% PFA in room temperature and stained using a BRCA1 monoclonal antibody (Product # MA1-23164)at a dilution of 1:400. Nuclei were stained using DAPI. Detection was performed using an Alexa Fluor-488 secondary antibody.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748.|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay-dependent|
|Immunohistochemistry (IHC)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
MA1-23164 detects BRCA1 in Human and Mouse samples.
MA1-23164 has been successfully used in chromatin immunoprecipitation, immunohistochemistry, immunocytochemistry, immunofluorescence, Immunoprecipitation and Western Blot procedures. The predicted molecular weight is 220 kDa. A suggested positive control for this product is HBL100.
MA1-23164 immunogen corresponds to BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748.
Store at 4°C short term. For extended storage, store at -20°C or below, avoiding freeze/thaw cycles.
BRCA1-6B4 recognizes full length BRCA1, a 220kDa nuclear phosphoprotein, and does not recognize the exon 11 splice variant. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Recent studies suggest a role for BRCA1 in DNA double strand break repair because of its association with Rad51.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Analysis of alternative lengthening of telomere markers in BRCA1 defective cells.
MA1-23164 was used in immunocytochemistry and western blot to analyze telomere markers and alternative lengthening in BRCA1 defective cells
|Kargaran PK,Yasaei H,Anjomani-Virmouni S,Mangiapane G,Slijepcevic P||Genes, chromosomes and cancer (55:864)||2016|