|ChIP assay (ChIP)||Assay-dependent|
|Immunohistochemistry (IHC)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1000|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748.|
|Storage buffer||PBS, pH 7.2|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA1-23164 detects BRCA1 in Human and Mouse samples.
MA1-23164 has been successfully used in chromatin immunoprecipitation, immunohistochemistry, immunocytochemistry, immunofluorescence, Immunoprecipitation and Western Blot procedures. The predicted molecular weight is 220 kDa. A suggested positive control for this product is HBL100.
MA1-23164 immunogen corresponds to BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748.
Store at 4°C short term. For extended storage, store at -20°C or below, avoiding freeze/thaw cycles.
BRCA1-6B4 recognizes full length BRCA1, a 220kDa nuclear phosphoprotein, and does not recognize the exon 11 splice variant. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Recent studies suggest a role for BRCA1 in DNA double strand break repair because of its association with Rad51.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: BRCA1/BRCA2-containing complex, subunit 1; breast and ovarian cancer susceptibility protein 1; breast and ovarian cancer sususceptibility protein 1; Breast Cancer 1; Breast Cancer 1 Early Onset; breast cancer 1, early onset; breast cancer associated 1; Breast cancer type 1 susceptibility protein; Breast cancer type 1 susceptibility protein homolog; Breast Ovarian Cancer Susceptibility; early onset breast cancer 1; Fanconi anemia, complementation group S; IRIS; Papillary Serous Carcinoma Of The Peritoneum; protein phosphatase 1, regulatory subunit 53; PSCP; RING finger protein 53; RING-type E3 ubiquitin transferase BRCA1; RNF53; truncated BRCA1; truncated breast and ovarian cancer susceptibility protein 1; truncated breast and ovarian cancer sususceptibility protein 1; truncated breast cancer type 1 susceptibility protein
Gene Aliases: BRCA1; BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4; PPP1R53; PSCP; RNF53
Molecular Function: ubiquitin-protein ligase
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