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|Tested species reactivity||Eubacteria|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Full length E. coli protein.|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26871 detects bacterial Alkaline Phosphatase from bacteria samples. PA1-26871 will not react with mammalian forms of Alkaline Phosphatase.
PA1-26871 has been successfully used in immunohistochemistry (frozen), immunomicroscopy, and Western blot procedures.
The PA1-26871 immunogen is full length E. coli protein.
Arg22 is removed from the mature enzyme by Iap to form isozyme 3. [More information is available at EcoGene: EG10727]. Alkaline phosphatase is a dimer that occurs in three forms, depending upon the growth conditions of the cell. [More information is available at EcoCyc: EG10727].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.