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|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Mucin from ovarian cyst fluid.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||5 - 10 µg/ml|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 1 publications below|
A suggested positive control for this product is colorectal adenocarcinoma.
The antibody 2-25LE reacts with Lewisb blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
High-throughput carbohydrate microarray profiling of 27 antibodies demonstrates widespread specificity problems.
MA1-19346 was used in ELISA to study the specificity of a range of antiglycan antibodies
|Manimala JC,Roach TA,Li Z,Gildersleeve JC||Glycobiology (17:17C)||2007|
gastric mucin; lewis B blood group antigen; major airway glycoprotein; mucin 5, subtypes A and C, tracheobronchial/gastric; mucin 5AC, oligomeric mucus/gel-forming pseudogene; mucin-5 subtype AC, tracheobronchial; tracheobronchial mucin
leB; MUC5; MUC5AC; mucin; TBM