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|Tested species reactivity||Chemical|
|Published species reactivity||Rat, Fish|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||BrdU conjugated to BSA|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12502 targets Bromodeoxyuridine in IHC (P) applications and shows reactivity with All samples.
The MA5-12502 immunogen is brdU conjugated to BSA.
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. BrdU can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Treatment of Glucocorticoids Inhibited Early Immune Responses and Impaired Cardiac Repair in Adult Zebrafish.
MA5-12502 was used in immunocytochemistry to study the inhibitory effects of glucocorticoid treatment on adult zebra fish early immune responses and cardiac repair
|Huang WC,Yang CC,Chen IH,Liu YM,Chang SJ,Chuang YJ||PloS one (8:null)||2016|
Exogenous glucagon-like peptide-2 (GLP-2) prevents chemotherapy-induced mucositis in rat small intestine.
MA5-12502 was used in immunohistochemistry to study the effect of exogenous glucagon-like peptide-2 on chemotherapy-induced mucositis in rat small intestine
|Kissow H,Viby NE,Hartmann B,Holst JJ,Timm M,Thim L,Poulsen SS||Cancer chemotherapy and pharmacology (70:39)||2012|