|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids 50-100 of human C/EBP- gamma|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This antibody detects endogenous protein at a molecular weight of 22 kDa.
Purity is >95% by SDS-PAGE.
The transcription factor C/EBP alpha (CCAAT-enhancer binding protein) is a heatstable, sequence-specific DNA-binding protein first purified from rat liver nuclei that binds avidly to several different cis-regulatory DNA sequences commonly associated with viral and cellular genes transcribed by RNA polymerase II. C/EBP alpha regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP alpha uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic alpha helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP alpha appears to function exclusively in terminally differentiated, growth-arrested cells. Additional family members include C/EBP beta, C/EBP gamma,C/EBP delta and C/EBP epsilon, all of which exhibit similar DNA-binding specificities and affinities to C/EBP alpha. Furthermore, C/EBP beta and C/EBP delta readily form heterodimers both with each other as well as with C/EBP alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: C/EBP gamma; CCAAT/enhancer binding protein (C/EBP), gamma; CCAAT/enhancer binding protein ,gamma; CCAAT/enhancer-binding protein gamma; CEBP gamma; CEBPG; G-CSF promoter element 1 binding protein; GPE1-binding protein; GPE1-BP; GPE1-BP (C/EBP gamma); Granulocyte colony-stimulating factor promoter element 1-binding protein; homolog to a human CCAAT/enhancer binding protein - gamma; IG/EBP-1; Immunoglobulin enhancer-binding protein 1
Gene Aliases: C/EBP; C/EBP[g]; C/EBPgamma; CEBPG; CEBPRNA; GPE1-BP; GPE1BP; Ig/EBP; IG/EBP-1
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