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C5aR2 (extracellular) Antibody (AAR-033-200UL) in WB
Western blot analysis of human MEG-O1 chronic myelogenous leukemia cell lines lysate (lanes 1 and 3) and human K562 erythroleukemia cell line lysate (lanes 2 and 4): - 1,2. Anti-Human C5aR2 (extracellular) Antibody (#AAR-033), (1:400).3,4. Anti-Human C5aR2 (extracellular) Antibody , preincubated with Human C5aR2 (extracellular) Blocking Peptide (#BLP-AR033).
Product Details
AAR-033-200UL
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)DSVSYEYGDYSDLSD, corresponding to amino acid residues 4-18 of human C5a anaphylatoxin chemotactic receptor 2, Extracellular, N-terminus
Conjugate
Form
Lyophilized
Concentration
0.85 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 1 X 25 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
C5AR2 is an orphan member that belongs to anaphylatoxin receptor family. It is a seven transmembrane G-protein-coupled receptor consisting of an N-terminal N-glycosylation site, several conserved cysteines and several potential C-terminal phosphorylation sites. It binds to C5a and C5a-des-Arg and serves as a highly regulated scavenger receptor with a negative modulatory influence on C5aR-mediated inflammation. Northern Blot analysis suggests it is co- expressed along with C5aR in different tissues and cells including neutrophils/macrophages, mast cells, dendritic cells, brain, lung, heart, kidney, liver, ovary and testis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C5a anaphylatoxin chemotactic receptor 2; C5a anaphylatoxin chemotactic receptor C5L2; Complement component 5a receptor 2; G protein-coupled receptor 77; G protein-coupled receptor C5L2; G-protein coupled receptor 77
Gene Aliases: C5AR2; C5L2; GPF77; GPR77
UniProt ID: (Human) Q9P296
Entrez Gene ID: (Human) 27202
chemotaxis
inflammatory response
G-protein coupled receptor signaling pathway
regulation of complement activation
negative regulation of tumor necrosis factor production
positive regulation of epithelial cell proliferation
positive regulation of ERK1 and ERK2 cascade
negative regulation of neutrophil chemotaxis
negative regulation of interleukin-6 secretion
regulation of interleukin-8 secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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