PA3-026 detects calcineurin inhibitor (cain) from human and rat tissues.
PA3-026 has been successfully used in Western blot, immunoprecipitation, immunofluorescence, and ELISA procedures. By Western blot, this antibody detects an ~240 kDa protein representing cain from rat liver extract. Immunoprecipitation of cain from rat brain tissues shows an interaction between cain and amphiphysin 1, adaptin, and dynamin 1.
The PA3-026 immunizing peptide is a His-tagged fusion protein corresponding to amino acid residues 1881-2173 from rat cain.
Calcineurin is a widely distributed Ser/Thr, calcium and calmodulin-dependent protein phosphatase. It mediates the immunosuppressive actions of drugs such as FK506 and cyclosporin, and has also been implicated in a number of calcium-sensitive pathways in the nervous system. Calcineurin has been found to be associated with other proteins, such as calmodulin, FKBP12, the ryanodine receptor, inositol 1,4,5-trisphosphate, and a recently identified protein, calcineurin inhibitor (cain).
Cain has been shown to bind calcineurin through its C-terminal tail. Recent studies have shown cain to bind to both amphiphysin 1 and calcineurin simultaneously and therefore acts as a physiological inhibitor of calcineurin. The binding of cain to amphiphysin 1 does not affect the interaction of amphiphysin 1 with other endocytic proteins. Cain, like calcineurin, is highly expressed in neurons and other tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 214 kDa nucleoporin; Calcineurin Inhibitor; CAN protein, putative oncogene; Nuclear pore complex protein Nup214; nucleoporin 214kDa; Nucleoporin Nup214; Protein CAN
Gene Aliases: CAIN; CAN; KIAA0023; NUP214; RGD1304977
UniProt ID: (Human) P35658