|Tested species reactivity||Bovine, Human, Mouse, Rabbit, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rat / IgG2a|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Gel Shift (GS)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:250-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
In Western blot, this antibody detects a band at ~60 kD in lysates of heat-shocked Hela cells.
This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Exploring the interactome: microfluidic isolation of proteins and interacting partners for quantitative analysis by electron microscopy.
MA1-82643 was used in immunoprecipitation to develop a microfluidic method for the isolation of native protein complexes for electron microscopic analysis
|Giss D,Kemmerling S,Dandey V,Stahlberg H,Braun T||Analytical chemistry (86:4680)||2014|