Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452) and 0.125 µg of Armenian Hamster IgG Isotype Control PerCP-Cyanine5-5 (Product # 45-4888) (left) or 0.125 µg of Anti-Mouse CD11c PerCP-Cyanine5-5 (right). Total viable cells were used for analysis.
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Armenian hamster / IgG|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.25 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Description: The N418 monoclonal antibody reacts with mouse CD11c, the integrin alpha X. CD11c non-covalently associates with beta 2 integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, a subset of Intestinal Intraepithelial Lymphocytes (IEL) and some activated T cells. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer.
Applications Reported: This N418 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This N418 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser
ITGAX is the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
||Induction of robust immune responses against human immunodeficiency virus is supported by the inherent tropism of adeno-associated virus type 5 for dendritic cells.||Xin KQ,Mizukami H,Urabe M,Toda Y,Shinoda K,Yoshida A,Oomura K,Kojima Y,Ichino M,Klinman D,Ozawa K,Okuda K||Journal of virology (80:11899)||2006|
|Not Applicable||Not Cited||Induction of robust immune responses against human immunodeficiency virus is supported by the inherent tropism of adeno-associated virus type 5 for dendritic cells.||Xin KQ,Mizukami H,Urabe M,Toda Y,Shinoda K,Yoshida A,Oomura K,Kojima Y,Ichino M,Klinman D,Ozawa K,Okuda K||Journal of virology (80:11899)||2006|
|Not Applicable||Not Cited||Essential roles of DC-derived IL-15 as a mediator of inflammatory responses in vivo.||Ohteki T,Tada H,Ishida K,Sato T,Maki C,Yamada T,Hamuro J,Koyasu S||The Journal of experimental medicine (203:2329)||2006|
|Not Applicable||Not Cited||The existence of CD11c+ sentinel and F4/80+ interstitial dendritic cells in dental pulp and their dynamics and functional properties.||Zhang J,Kawashima N,Suda H,Nakano Y,Takano Y,Azuma M||International immunology (18:1375)||2006|