Invitrogen

CD11c Monoclonal Antibody (N418), Super Bright™ 600, eBioscience™

Product Image Gallery

CD11c Antibody in Flow Cytometry (Flow) — C57BL/6 mouse splenocytes were stained with CD45R (B220) Monoclonal Antibody, FITC (Product # 11-0452-82) and 0.06 µg of Armenian Hamster IgG Isotype Control, Super Bright 600 (Product # 63-4888-82) (left) or 0.06 µg of CD11c Monoclonal Antibody, Super Bright 600 (right). Total viable cells were used for analysis.

CD11c Antibody in Immunocytochemistry (ICC/IF) — FLCs are different from macrophages. (A) Cell morphology of mouse FLCs at passage 2 (right panel) and macrophage cell line Raw 264.7 (left panel). (B) FLCs at passage 1 and Raw264.7 were stained with either CD11b or CD11c or CD14 antibody. Scale bars, 50 mum.

CD11c Antibody in Immunohistochemistry (IHC) — Figure 6 Glufosinate treatment promotes M1-like TAMs, CTL tumor accumulation, vessel pruning, and normalization and inhibits metastasis in a murine model of melanoma A Experimental plan. Mice were intradermally injected with YUMM 1.7 (CD90.1 + ) cells. When the tumor reached 80-100 mm 3 size, mice were treated by gavage with glufosinate (20 mg/kg) or vehicle for 14 days. B, C Intradermal YUMM 1.7 (CD90.1 + ) tumor growth over the time (B) and number of metastatic lesions per lung (C) in vehicle and glufosinate (20 mg/kg)-treated mice (pool of 2 independent experiments; 10 mice per condition in total). D Representative images of metastatic lesions checked by DAB-H stain for CD90.1 positivity in vehicle and glufosinate (20 mg/kg)-treated mice ( n = 6). Six images per lung were analyzed. Scale bar: 2 mm. Zoomed area scale bar: 50 mum. E, F FACS quantification of M1-like MHC class II high (E) and MHC class II low (F) CD11c + CD206 - TAMs over the total number of F4/80 + cells in vehicle and glufosinate (20 mg/kg)-treated mice ( n = 6). G Quantification and representative images of CD11c + cells over the total number of F4/80 + cells in vehicle and glufosinate (20 mg/kg)-treated mice ( n = 6). Six images per tumor were analyzed. Scale bars: 50 mum. H, I FACS quantification of M2-like MHC class II high (H) and MHC class II low (I) CD11c - CD206 + TAMs over the total number of F4/80 + cells in vehicle and glufosinate (20 mg/kg)-treated mice ( n = 6). J Quantification and representat

CD11c Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow) — Glufosinate treatment promotes M1-like TAMs, CTL tumor accumulation, vessel pruning, and normalization and inhibits metastasis in a murine model of melanoma A Experimental plan. Mice were intradermally injected with YUMM 1.7 (CD90.1 + ) cells. When the tumor reached 80-100 mm 3 size, mice were treated by gavage with glufosinate (20 mg/kg) or vehicle for 14 days. B, C Intradermal YUMM 1.7 (CD90.1 + ) tumor growth over the time (B) and number of metastatic lesions per lung (C) in vehicle and glufosinate (20 mg/kg)-treated mice (pool of 2 independent experiments; 10 mice per condition in total). D Representative images of metastatic lesions checked by DAB-H stain for CD90.1 positivity in vehicle and glufosinate (20 mg/kg)-treated mice ( n = 6). Six images per lung were analyzed. Scale bar: 2 mm. Zoomed area scale bar: 50 mum. E, F FACS quantification of M1-like MHC class II high (E) and MHC class II low (F) CD11c + CD206 - TAMs over the total number of F4/80 + cells in vehicle and glufosinate (20 mg/kg)-treated mice ( n = 6). G Quantification and representative images of CD11c + cells over the total number of F4/80 + cells in vehicle and glufosinate (20 mg/kg)-treated mice ( n = 6). Six images per tumor were analyzed. Scale bars: 50 mum. H, I FACS quantification of M2-like MHC class II high (H) and MHC class II low (I) CD11c - CD206 + TAMs over the total number of F4/80 + cells in vehicle and glufosinate (20 mg/kg)-treated mice ( n = 6). J Quantification and representative image

Product Details

63-0114-82

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 2 publications 2 publications
Flow Cytometry (Flow)	0.125 µg/test	View 10 publications 10 publications

Product Specifications
Species Reactivity	Mouse
Published species	Not Applicable
Host/Isotype	Armenian hamster / IgG
Recommended Isotype Control	Armenian Hamster IgG Isotype Control (eBio299Arm), Super Bright™ 600, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	N418
Conjugate	Super Bright™ 600 Alexa Fluor 488 Alexa Fluor 532 Alexa Fluor 660 Alexa Fluor 700 APC APC-eFluor 780 Biotin Brilliant UV 737 Brilliant UV 805 eFluor 450 eFluor 506 eFluor 615 eFluor 660 FITC Functional Grade NovaFluor Blue 510 NovaFluor Blue 610-30S NovaFluor Blue 660-40S NovaFluor Yellow 570 PE PE-Cyanine5 PE-Cyanine5.5 PE-Cyanine7 PE-eFluor 610 PerCP-Cyanine5.5 Super Bright
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2722930

Product Specific Information

Description: The N418 monoclonal antibody reacts with mouse CD11c, the integrin alphaX. CD11c non-covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, a subset of Intestinal Intraepithelial Lymphocytes (IEL) and some activated T cells. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer.

Applications Reported: This N418 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This N418 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD11, along with CD18, form a heterodimer adhesion molecule. In particular, CD11 is composed of CD11a, CD11b and CD11c. CD11a is a leukocyte marker that is expressed in B and T lymphocytes, macrophages, monocytes, neutrophils, basophils and eosinophils. CD11b is primarily expressed by monocytes, macrophages, natural killer cells, some B and T-cells, and granulocytes. CD11c is expressed in monocytes, macrophages, natural killer cells, some granulocytes and less so in a subset of lymphocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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