Invitrogen

CD16 Monoclonal Antibody (eBioCB16 (CB16)), Biotin, eBioscience™

Product Image Gallery

CD16 Antibody in Flow Cytometry (Flow) — Staining of normal human peripheral blood cells with 0.06 µg of Mouse IgG1 K Isotype Control Biotin (Product # 13-4714-85) (open histogram) or 0.06 µg of Anti-Human CD16 Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte (left) or granulocyte (right) gates were used for analysis.

CD16 Antibody in Immunocytochemistry (ICC/IF) — Figure 3 Construction of the PVAX1-HPV58 mE6E7FcGB plasmid and expression of the target proteins assessed by immunofluorescence staining. Notes: ( A ) Diagram of the construction of the recombinant DNA vaccine PVAX1-HPV58 mE6E7FcGB. HPV58 mE6E7 was cloned into the plasmid PCI-sig-Fc-GPI, and the fused cassette sig-HPV58 mE6E7-Fc-GPI was subsequently excised from PCI-sig-HPV58 mE6E7-Fc-GPI plasmid and inserted into the upstream of IRES in PVAX1-IRES-GM/B7.1 vector to create PVAX1-HPV58 mE6E7FcGB. ( B ) The transfected 293T cells were concurrently incubated with a rabbit anti-human IgG-FITC antibody (green) and a mouse anti-human B7-1-PE antibody (red). Immunofluorescence revealed the expression of sig-HPV58 mE6E7-Fc-GPI (a, green, x50) and GM-CSF/B7.1 (b, red, x50) at the cells membrane. Abbreviations: IgG-FITC, immunoglobulin G-conjugated fluorescein isothiocyanate; IRES, internal ribosome entry site; HPV, human papillomavirus.

CD16 Antibody in T-Cell Activation (TCA) — Fig. 3 HIV-specific responses and immune response capability of the case at 9.5 years of age. a Detection of HIV-specific antibodies at 9.5 years of age by western blot. The case antibody profile is compared with controls that are a high positive, low positive and HIV-negative. HIV proteins corresponding to bands in the blots are shown in the left grey-shaded block; the case profile was positive for the core proteins indicated in pink. b Quantitation of HIV-specific antibodies by multiplex bead array for all isotypes and subclasses (indicated on the left side--IgG1, IgG2, IgG3, IgG4, IgM, IgA1, IgA2) against gp41, Gag, RT, gp120, Nef, Tat, Vpu, peptide Vpu9 and V1V2 scaffold antigens (indicated at the top). Results are expressed as mean fluorescence intensities (MFI)), the colour key shows ranges of MFI according to colour intensity (the darker the more HIV-specific antibody detected). A result is considered positive if above the cut-off (mean +- 3 SD) determined from eight adult uninfected controls. Vpu9 amino acid sequence: STMVDMGHLRLLDVNDL. c Proportions of natural killer (NK) cells that respond to anti-CD16 antibody, and CD4+ and CD8+ T cells that respond to staphylococcal enterotoxin B (SEB) in a whole blood intracellular cytokine (ICC) assay that measures induction of interferon-gamma (IFN-gamma) and interleukin-2 (IL-2). HIV-uninfected adult reference values for comparison ( n = 21; median % and range)--natural killer (NK) anti-CD16%: 37.92 (12-67.6), CD4 SEB%:

CD16 Antibody — Staining of human peripheral blood cells. As expected based on known relative expression patterns, CD16 clone CB16 stains all granulocytes, a subset of monocytes and a subset of lymphocytes (NK cells). Details: Normal human whole blood was surface stained with CD16 (clone CB16). After staining, red blood cells were lysed using 1-step Fix/Lyse Buffer. Cells in the lymphocyte (purple histogram), monocyte (orange histogram), or granulocyte (blue histogram) gates were used for analysis. {RE}

Product Details

13-0168-82

Applications	Tested Dilution	Publications
Immunocytochemistry (ICC/IF)	-	View 2 publications 2 publications
Flow Cytometry (Flow)	0.125 µg/test	View 14 publications 14 publications
T-Cell Activation (TCA)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Not Applicable
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	eBioCB16 (CB16)
Conjugate	Biotin Alexa Fluor 700 APC APC-eFluor 780 Brilliant UV 661 Brilliant UV 737 Brilliant UV 805 Brilliant Violet 480 eFluor 450 eFluor 506 FITC Functional Grade NovaFluor Blue 610-70S NovaFluor Blue 660-120S NovaFluor Red 710 NovaFluor Red 725 NovaFluor Yellow 660 NovaFluor Yellow 730 PE PE-Cyanine5 PE-Cyanine7 PE-eFluor 610 PerCP-eFluor 710 Super Bright 436 Super Bright 600 Super Bright 645
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_529489

Product Specific Information

Description: The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. CD16 is represented by two similar genes, CD16A (Fc gammaRIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fc gammaRIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.

Applications Reported: This eBioCB16 (CB16) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioCB16 (CB16) antibody has been tested by flow cytometric analysis of normal normal human peripheral blood cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD16 (FCGR3A) is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Human CD16 is expressed in two forms – FCGR3A and FCGR3B. FCGR3A is associated with the FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FCGR3A is associated, with FcepsilonRI-beta subunit. Besides IgG, FCGR3A can be triggered also by oligomeric IgE. FCGR3B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a pro-adhesive phenotype. Diseases associated with CD16 dysfunction include immunodeficiency 20 and systemic lupus erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

-------A type I IFN, prothrombotic hyperinflammatory neutrophil signature is distinct for COVID-19 ARDS--.

Wellcome open research

Reyes L,A Sanchez-Garcia M,Morrison T,Howden AJM,Watts ER,Arienti S,Sadiku P,Coelho P,Mirchandani AS,Zhang A,Hope D,Clark SK,Singleton J,Johnston S,Grecian R,Poon A,McNamara S,Harper I,Fourman MH,Brenes AJ,Pathak S,Lloyd A,Blanco GR,von Kriegsheim A,Ghesquiere B,Vermaelen W,Cologna CT,Dhaliwal K,Hirani N,Dockrell DH,Whyte MKB,Griffith D,Cantrell DA,Walmsley SR

Published figure using CD16 monoclonal antibody (Product # 13-0168-82) in Flow Cytometry

Wed Jul 20 00:00:00 EDT 2022

Prognostic Lnc-S100B-2 Affects Cell Apoptosis and Microenvironment of Colorectal Cancer through MLLT10 Signaling.

Journal of oncology

Yi J,Peng F,Zhao J,Gong X

Published figure using CD16 monoclonal antibody (Product # 13-0168-82) in Flow Cytometry

Tue Feb 08 00:00:00 EST 2022

Isolation of polymorphonuclear neutrophils and monocytes from a single sample of human peripheral blood.

STAR protocols

Cui C,Schoenfelt KQ,Becker KM,Becker L

Published figure using CD16 monoclonal antibody (Product # 13-0168-82) in Flow Cytometry

Fri Dec 17 00:00:00 EST 2021

Circulating Neutrophils of Nonalcoholic Steatohepatitis Patients Show an Activated Phenotype and Suppress T Lymphocytes Activity.

Journal of immunology research

Antonucci L,Porcu C,Timperi E,Santini SJ,Iannucci G,Balsano C

Published figure using CD16 monoclonal antibody (Product # 13-0168-82) in Flow Cytometry

Fri May 21 00:00:00 EDT 2021

Broad and potent neutralizing human antibodies to tick-borne flaviviruses protect mice from disease.

The Journal of experimental medicine

Agudelo M,Palus M,Keeffe JR,Bianchini F,Svoboda P,Salát J,Peace A,Gazumyan A,Cipolla M,Kapoor T,Guidetti F,Yao KH,Elsterová J,Teislerová D,Chrdle A,Hönig V,Oliveira T,West AP,Lee YE,Rice CM,MacDonald MR,Bjorkman PJ,Růžek D,Robbiani DF,Nussenzweig MC

Published figure using CD16 monoclonal antibody (Product # 13-0168-82) in Flow Cytometry

Mon May 03 00:00:00 EDT 2021

SARS-CoV-2-triggered neutrophil extracellular traps mediate COVID-19 pathology.

The Journal of experimental medicine

Veras FP,Pontelli MC,Silva CM,Toller-Kawahisa JE,de Lima M,Nascimento DC,Schneider AH,Caetité D,Tavares LA,Paiva IM,Rosales R,Colón D,Martins R,Castro IA,Almeida GM,Lopes MIF,Benatti MN,Bonjorno LP,Giannini MC,Luppino-Assad R,Almeida SL,Vilar F,Santana R,Bollela VR,Auxiliadora-Martins M,Borges M,Miranda CH,Pazin-Filho A,da Silva LLP,Cunha LD,Zamboni DS,Dal-Pizzol F,Leiria LO,Siyuan L,Batah S,Fabro A,Mauad T,Dolhnikoff M,Duarte-Neto A,Saldiva P,Cunha TM,Alves-Filho JC,Arruda E,Louzada-Junior P,Oliveira RD,Cunha FQ

Published figure using CD16 monoclonal antibody (Product # 13-0168-82) in Flow Cytometry

Mon Dec 07 00:00:00 EST 2020

CD14⁺CD16^- monocytes are the main precursors of osteoclasts in rheumatoid arthritis via expressing Tyro3TK.

Arthritis research & therapy

Xue J,Xu L,Zhu H,Bai M,Li X,Zhao Z,Zhong H,Cheng G,Li X,Hu F,Su Y

Published figure using CD16 monoclonal antibody (Product # 13-0168-82) in Flow Cytometry

Mon Sep 21 00:00:00 EDT 2020

IL-36 Promotes Systemic IFN-I Responses in Severe Forms of Psoriasis.

The Journal of investigative dermatology

Catapano M,Vergnano M,Romano M,Mahil SK,Choon SE,Burden AD,Young HS,Carr IM,Lachmann HJ,Lombardi G,Smith CH,Ciccarelli FD,Barker JN,Capon F

Published figure using CD16 monoclonal antibody (Product # 13-0168-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Loss of testosterone impairs anti-tumor neutrophil function.

Nature communications

Markman JL,Porritt RA,Wakita D,Lane ME,Martinon D,Noval Rivas M,Luu M,Posadas EM,Crother TR,Arditi M

Published figure using CD16 monoclonal antibody (Product # 13-0168-82) in Flow Cytometry

Tue Mar 31 00:00:00 EDT 2020

A child with perinatal HIV infection and long-term sustained virological control following antiretroviral treatment cessation.

Nature communications

Violari A,Cotton MF,Kuhn L,Schramm DB,Paximadis M,Loubser S,Shalekoff S,Da Costa Dias B,Otwombe K,Liberty A,McIntyre J,Babiker A,Gibb D,Tiemessen CT

Published figure using CD16 monoclonal antibody (Product # 13-0168-82) in T-Cell Activation

Thu Jan 24 00:00:00 EST 2019

Ex vivo culture of human atherosclerotic plaques: A model to study immune cells in atherogenesis.

Atherosclerosis

Lebedeva A,Vorobyeva D,Vagida M,Ivanova O,Felker E,Fitzgerald W,Danilova N,Gontarenko V,Shpektor A,Vasilieva E,Margolis L

Published figure using CD16 monoclonal antibody (Product # 13-0168-82) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2017

Metformin-treated cancer cells modulate macrophage polarization through AMPK-NF-κB signaling.

Oncotarget

Chiang CF,Chao TT,Su YF,Hsu CC,Chien CY,Chiu KC,Shiah SG,Lee CH,Liu SY,Shieh YS

Published figure using CD16 monoclonal antibody (Product # 13-0168-82) in Flow Cytometry

Tue Mar 28 00:00:00 EDT 2017

Pulmonary sarcoidosis is associated with high-level inducible co-stimulator (ICOS) expression on lung regulatory T cells--possible implications for the ICOS/ICOS-ligand axis in disease course and resolution.

Clinical and experimental immunology

Sakthivel P,Grunewald J,Eklund A,Bruder D,Wahlström J

Published figure using CD16 monoclonal antibody (Product # 13-0168-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

A DNA vaccine encoding mutated HPV58 mE6E7-Fc-GPI fusion antigen and GM-CSF and B7.1.

OncoTargets and therapy

Wang H,Yu J,Li L

Published figure using CD16 monoclonal antibody (Product # 13-0168-82) in Immunofluorescence

Wed Nov 25 00:00:00 EST 2015

Retinoic acid regulates hematopoietic development from human pluripotent stem cells.

Stem cell reports

Rönn RE,Guibentif C,Moraghebi R,Chaves P,Saxena S,Garcia B,Woods NB

Published figure using CD16 monoclonal antibody (Product # 13-0168-82) in Flow Cytometry

Tue Feb 10 00:00:00 EST 2015

Decreased plasma levels of soluble CD18 link leukocyte infiltration with disease activity in spondyloarthritis.

Arthritis research & therapy

Kragstrup TW,Jalilian B,Hvid M,Kjærgaard A,Østgård R,Schiøttz-Christensen B,Jurik AG,Robinson WH,Vorup-Jensen T,Deleuran B

Published figure using CD16 monoclonal antibody (Product # 13-0168-82) in Immunofluorescence

Tue Feb 04 00:00:00 EST 2014

Changes in natural killer cell subsets in pediatric liver transplant recipients.

Pediatric transplantation

Pham B,Piard-Ruster K,Silva R,Gallo A,Esquivel CO,Martinez OM,Krams SM

Published figure using CD16 monoclonal antibody (Product # 13-0168-82) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: CD16-I; CD16-II; CD16a; CD16a antigen; CD16b; CD32 receptor 2; Fc fragment of IgG low affinity IIIa receptor; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc gamma receptor III-A; Fc gamma receptor IIIa; Fc gamma receptor IIIb; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RIII; Fc-gamma RIII-alpha; Fc-gamma RIII-beta; Fc-gamma RIIIa; fc-gamma RIIIb; FCG2; FcgammaRIIIA; FCGR2; FCGR2A; FCGR2B; FCGR2C; FcR-10; FcRII-b; FcRII-c; IgG Fc receptor III-1; IgG Fc receptor III-2; IgG Fc receptor III-A; immunoglobulin G Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor III-A; Low affinity immunoglobulin gamma Fc region receptor III-B; neutrophil-specific antigen NA; RP11-5K23.1

Gene Aliases: CD16; CD16A; CD16B; FCG3; FCGR3; FCGR3A; FCGR3B; FCGRIII; FCR-10; FCRIII; FCRIIIA; FCRIIIb; IGFR3; IMD20

UniProt ID: (Human) P08637, (Human) O75015

Entrez Gene ID: (Human) 2214, (Human) 2215

Molecular Function: immunoglobulin receptor superfamily