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Invitrogen
CD195 (CCR5) Monoclonal Antibody (HM-CCR5 (7A4)), APC, eBioscience™
FIGURE: 1 / 2
CD195 (CCR5) Antibody (17-1951-82) in Flow
Staining of FcR-blocked (Anti-Mouse CD16/CD32 Purified, Product # 14-0161-82) C57BL/6 mouse thioglycolate-elicited peritoneal exudate cells with CD11b Monoclonal Antibody, FITC (Product # 11-0112-82) and 1.0 µg of Armenian Hamster IgG Isotype Control, APC (Product # 17-4888-82) (left) or 1.0 µg of CD195 Monoclonal Antibody, APC (right). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
Product Details
17-1951-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Armenian hamster
/ IgG
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
HM-CCR5 (7A4)
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2802223
Product Specific Information
Description: CCR5 is the major co-receptor for macrophage-tropic isolates of HIV-1. CCR5, also known as CD195, is a seven transmembrane chemokine receptor capable of binding RANTES, MIP-1 alpha and MIP-1 beta. Knockout mice show a decreased recruitment of macrophages and increased susceptibility to infection. Protein and mRNA has been found in macrophages from thioglycolate-treated and activated T cells.
The HM-CCR5(7A4) monoclonal antibody binds the N terminal extracellular domain of mouse CCR5 with no crossreactivity to human CCR5.
Applications Reported: This HM-CCR5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HM-CCR5 antibody has been tested by flow cytometric analysis of thioglycolate-induced peritoneal exudate cells. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
Target Information
Human immunodeficiency virus (HIV) and related virus require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CCR5 (CC-CKR-5) is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains fusion and entry of human white blood cells. CCR5 is required for the infection by HIV-1, HIV-2, and SIV. The beta-chemokines RANTES, MIP-alpha and MIP-beta are the ligands for CCR5 and prevent infection by M-tropic HIV-1. CCR5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells. The amino-terminal domain and the extracellular loops of CCR5 serve as HIV biding sites. CCR5 messenger RNA is expressed in lymphoid organs and monocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C chemokine receptor type 5; C-C CKR-5; CC-CKR-5; CCR-5; CD195; CD195 antigen; chemokine (C-C) receptor 5; MIP-1 alpha receptor
Gene Aliases: AM4-7; Ccr5; CD195; Cmkbr5
UniProt ID: (Mouse) P51682
Entrez Gene ID: (Mouse) 12774
calcium ion transport
chemotaxis
defense response
inflammatory response
immune response
signal transduction
G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
cell-cell signaling
positive regulation of gene expression
release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
calcium-mediated signaling
positive regulation of cell-cell adhesion
negative regulation of cell migration
negative regulation of axon extension
positive regulation of fever generation
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
positive regulation of neuron differentiation
positive regulation of interleukin-1 beta secretion
positive regulation of inflammatory response
positive regulation of apoptotic process by virus
chemokine-mediated signaling pathway
response to cholesterol
neuron death
positive regulation of cell migration involved in sprouting angiogenesis
negative regulation of macrophage apoptotic process
negative regulation of neural precursor cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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