Invitrogen

CD206 (MMR) Monoclonal Antibody (MR6F3), PE-Cyanine7, eBioscience™

Product Image Gallery

CD206 (MMR) Antibody in Flow Cytometry (Flow) — Mouse resident peritoneal exudate cells were surface stained with Anti-Mouse F4/80 Antigen eFluor® 450 (Product # 48-4801-82) followed by fixation and permeabilization with the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Cells were then intracellularly stained with 0.125 µg of Rat IgG2b K Isotype Control PE-Cyanine7 (Product # 25-4031-82) (left) or 0.125 µg of Anti-Mouse CD206 (MMR) PE-Cyanine7 (right). Total cells were used for analysis.

Product Details

25-2061-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 31 publications 31 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MR6F3

Conjugate

PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_2637424

Product Specific Information

Description: This MR6F3 antibody recognizes mouse CD206 also known as Macrophage Mannose Receptor (MMR) or Mannose Receptor C, Type 1 (MRC1). CD206 is a 175-kDa, type 1 integral membrane glycoprotein receptor that is present in macrophages, some dendritic cells, as well as liver and lymphoid endothelial cells. CD206 belongs to the C-type lectin family. Extracellular regions of CD206 include an N-terminal cysteine-rich (CR) domain that binds sulfated glycoproteins, a fibronectin II (FNII) domain that binds collagens, and eight carbohydrate recognition domains (CRDs) that bind sugars like mannose and fucose with high affinity. CD206 mediates phagocytic and endocytic uptake of fungal, bacterial, protozoan and viral antigens, and plays an important role in immune defense and immune regulation. A soluble form of CD206 is generated by cleavage of the full-length protein, and it can be detected in in vitro macrophage cell culture supernatants and in mouse serum. CD206 is considered to be one of the markers of M2 macrophages. Factors inducing its expression include: IL-4, IL-13, M-CSF, IL-6, IL-10, and glucocorticoids, while TNF-alpha, IFN-gamma, TGF-beta, and LPS have been reported to down-regulate expression of CD206.

It has been demonstrated that only a small fraction of CD206 is present at the cell surface, therefore, intracellular staining is recommended.

Applications Reported: This MR6F3 antibody has been reported for use in flow cytometric analysis, and intracellular staining followed by flow cytometric analysis.

Applications Tested: This MR6F3 antibody has been tested by intracellular staining and flow cytometric analysis of mouse resident peritoneal exudate cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD206 (MSR, Mannose receptor, MRC1) is a 175 kDa transmembrane protein belonging to the group of pattern recognition receptors. CD206 is predominantly expressed in tissue macrophages, dendritic cells, a subpopulation of endothelial cells and sperm cells. CD206 is thought to play a role in the innate and adaptive immune response. CD206 is also expressed on microglia and mato cells of the brain but not astrocytes or neurons. CD206 also mediate the recognition and uptake of a variety of macromolecules, including modified lipoproteins, advanced glycation end (AGEs) products and amyloid b-protein (Abeta). While the normal role of CD206 is associated with cell adhesion and host defense mechanisms, it also has been implicated in the development of atherosclerosis and Amyloid beta deposition in Alzheimer's disease (AD). CD206's gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. CD206 has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages. Other diseases associated with CD206 dysfunction include leprosy and Gaucher's Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

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Li S,Zhang L,Liu C,Kim J,Su K,Chen T,Zhao L,Lu X,Zhang H,Cui Y,Cui X,Yuan F,Pan H

Published figure using CD206 (MMR) monoclonal antibody (Product # 25-2061-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2023

Caspase-11 promotes high-fat diet-induced NAFLD by increasing glycolysis, OXPHOS, and pyroptosis in macrophages.

Frontiers in immunology

Drummer C,Saaoud F,Jhala NC,Cueto R,Sun Y,Xu K,Shao Y,Lu Y,Shen H,Yang L,Zhou Y,Yu J,Wu S,Snyder NW,Hu W,Zhuo J',Zhong Y,Jiang X,Wang H,Yang X

25-2061-82 was used in Flow cytometry/Cell sorting to provide novel insights on the roles of the caspase-11-GSDMD pathway in promoting hepatic macrophage inflammation and pyroptosis and novel targets for future therapeutic interventions involving the transition of NAFLD to NASH, hyperlipidemia, type II diabetes, metabolic syndrome, metabolically healthy obesity, atherosclerotic cardiovascular diseases, autoimmune diseases, liver transplantation, and hepatic cancers.

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Thu Nov 03 00:00:00 EDT 2022

Mineralocorticoid receptor antagonists and glucocorticoids differentially affect skeletal muscle inflammation and pathology in muscular dystrophy.

JCI insight

Howard ZM,Gomatam CK,Rabolli CP,Lowe J,Piepho AB,Bansal SS,Accornero F,Rafael-Fortney JA

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Mon Oct 10 00:00:00 EDT 2022

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Journal of applied microbiology

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G9a promotes inflammation in Streptococcus pneumoniae induced pneumonia mice by stimulating M1 macrophage polarization and H3K9me2 methylation in FOXP1 promoter region.

Annals of translational medicine

Li Y,Li G,Zhang L,Li Y,Zhao Z

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Sun May 01 00:00:00 EDT 2022

Electronic Cigarette Exposure Enhances Lung Inflammatory and Fibrotic Responses in COPD Mice.

Frontiers in pharmacology

Han H,Peng G,Meister M,Yao H,Yang JJ,Zou MH,Liu ZR,Ji X

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Hyperoside Suppresses Renal Inflammation by Regulating Macrophage Polarization in Mice With Type 2 Diabetes Mellitus.

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Liu J,Zhang Y,Sheng H,Liang C,Liu H,Moran Guerrero JA,Lu Z,Mao W,Dai Z,Liu X,Zhang L

25-2061-82 was used in Flow Cytometry to study the therapeutic effects of hyperoside on murine diabetic nephropathy and the potential mechanisms responsible for its efficacy.

Thu Feb 10 00:00:00 EST 2022

Hyperoside Suppresses Renal Inflammation by Regulating Macrophage Polarization in Mice With Type 2 Diabetes Mellitus.

Frontiers in immunology

Liu J,Zhang Y,Sheng H,Liang C,Liu H,Moran Guerrero JA,Lu Z,Mao W,Dai Z,Liu X,Zhang L

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STING Activated Tumor-Intrinsic Type I Interferon Signaling Promotes CXCR3 Dependent Antitumor Immunity in Pancreatic Cancer.

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Polarization of Type 1 Macrophages Is Associated with the Severity of Viral Encephalitis Caused by Japanese Encephalitis Virus and Dengue Virus.

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Scientific reports

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Published figure using CD206 (MMR) monoclonal antibody (Product # 25-2061-82) in Flow Cytometry

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Bioinformatics

Protein Aliases: CD206; Macrophage mannose receptor 1; MMR

Gene Aliases: AW259686; CD206; MR; Mrc1

UniProt ID: (Mouse) Q61830

Entrez Gene ID: (Mouse) 17533

Function(s)

endocytosis receptor-mediated endocytosis signal transduction cellular response to lipopolysaccharide cellular response to interferon-gamma cellular response to interleukin-4

Process(es)

receptor activity transmembrane signaling receptor activity mannose binding carbohydrate binding

CD206 (MMR) Monoclonal Antibody (MR6F3), PE-Cyanine7, eBioscience™

Product Details

25-2061-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

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