Immunohistochemistry analysis of Glycophorin A showing staining in the membrane of paraffin-embedded human spleen tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Glycophorin A monoclonal antibody (Product # MA5-12484) diluted in 3% BSA-PBS at a dilution of 1:200 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:500|
|Western Blot (WB)||1:50-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
Glycophorin A is a sialoglycoprotein present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorins acts as the receptor for Sandei virus and parvovirus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Histopathological, immunophenotypic and clinical particularities and evolution of a case of hepatosplenic T-cell lymphoma in transformation to leukemia.
MA5-12484 was used in immunohistochemistry to report on a case of hepatosplenic T-cell lymphoma with leukemic transformation
|Benedek Lázár E,Köpeczi JB,Tunyogi AB,Kakucs E,Horváth E,Turcu M,Benedek I||Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie (54:1135)||2014|