Invitrogen

CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), Functional Grade, eBioscience™

Product Image Gallery

CD31 (PECAM-1) Antibody in Flow Cytometry (Flow) — Staining of normal human peripheral blood cells with Anti-Human CD31 (PECAM-1) FITC (left) or PE (right).Appropriate isotype controls were used (open histogram).Cells in the monocyte population were used for analysis.

CD31 (PECAM-1) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Figure 5. ProAgio eliminates angiogenic vessels, enhances blood perfusion into the tumor, and consequentially reduces hypoxia. (A-D) Representative images of IF CD31 staining (A), quantification of vessel number (B), number of branch points (C), and vessel length (D) per view field in tumor sections of 4T1 mice treated with vehicle or ProAgio. Nuclei were counterstained with DAPI (blue) . Scale bars, 100 um ( n = 6-7/group). (E and F) Representative IHC images of CD31 staining (E) and quantification of CD31 + area (F) in the breast tumor sections of MMTV-PyMT mice treated with vehicle or ProAgio. Insets, x1.5. Scale bars, 100 um ( n = 5/group). (G and H) Representative fluorescence images of lectin perfusion in tumor vessels (G) and quantification of lectin-positive area (red) per total CD31-positive area (H; green, represented by white arrows) in tumor sections of 4T1 mice treated with vehicle or ProAgio. Scale bars, 100 um ( n = 4/group). (I and J) Representative IF images of dextran leakage of tumor vessels (J) and quantification of dextran + area (I) in the breast tumor sections of 4T1 orthotopic mice. Dextran + area is presented as a percentage per total section of the CD31 + area. Scale bar, 100 um ( n = 4/group). (K) Intratumoral levels of PTX in the extracts of tumors of 4T1 mice treated with vehicle or ProAgio. PTX levels were presented as nanograms of PTX per milligrams of tumor tissue ( n = 4/group). (L and M) Representative fluorescence images of FITC-conjugated P

CD31 (PECAM-1) Antibody in Immunocytochemistry (ICC/IF) — Fig. 4 Functional properties of cardiac explant-derived cells. a Endothelial cells form capillary-like structures in Matrigel, uptake acLDL ( left ) and demonstrate a CD31-positive phenotype ( middle ). A merged image is represented in the picture on the right . Scale bar 100 mum. b Angiogenic cytokine profiles of conditioned medium obtained from cardiac explant-derived cells before and after MACS separation of CD31-positive cells. Wilcoxon test. c Evaluation of functional properties of CD31-positive endothelial cells in vivo. Visualization of injected mix (Matrigel + cells with the vital dye MitoTracker Deep Red FM) after 14 days with a Kodak In-Vivo Multispectral Imaging System device ( left ). Vasculature in cryosections of the Matrigel plug at day 14 after injection of Matrigel only, Matrigel + endothelial cells and Matrigel + endothelial + smooth muscle cells is detected by isolectin B4 Alexa 594 conjugate staining. Scale bar 100 mum. d A diagram representing the number of vessels positive for isolectin B4 Alexa 594 conjugate staining ( red colour ) as determined under a fluorescence microscope in 10 random fields of view. Wilcoxon test with Bonferroni correction. * vs Matrigel only, p = 0.0005; ** vs Matrigel + endothelial cells, p = 0.00003. e Fluorescent in situ hybridization of human and mouse DNA probes (hCot and mCot, respectively) to cryosection of Matrigel + EC + SMC. Human probes ( green ) are distributed evenly over the nuclei, whereas mouse probes ( red ) are

CD31 (PECAM-1) Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P)) — Figure 6. Immunohistochemical analysis of microvascular density and TUNEL assay in the mouse orthotopic tumor model. Tumor tissues were acquired at 20 days after the different treatments. (A) Monoclonal anti-CD31 antibody staining of the section to examine the microvascular density with x20 magnification, and (B) CD31-positive cells. Five sections were counted for each slide. (C) TUNEL assay was performed on the SMMC-7721-Fluc-induced orthotopic hepatocellular carcinoma model in mice with x20 magnification, and (D) the percentage of TUNEL-positive cells is presented. Values represent the mean +- standard error of the mean, and are representative of four independent experiments. *P<0.05, **P<0.01. HAL, hepatic artery ligation; Ctrl, control; LV, lentivirus.

CD31 (PECAM-1) Antibody in Immunohistochemistry (IHC) — Gel@MSN/miR-21-5p promoted local neovascularization at the infarct site after MI. ( A ) Micro-CT angiography analysis of 3D vascular structures within the infarct zone 28 days after MI indicates that the vascular volume was significantly increased in the Gel@MSN/miR-21-5p treatment group. The vascular volume within the infarct zone was quantitatively analyzed. * P < 0.05 and *** P < 0.01. n = 3 per group. ( B ) Immunofluorescence staining for CD31 (red) identified the vascular endothelium, and staining for alpha-SMA (green) identified myofibroblasts and pericytes, showing that the cardiac capillary density in histological sections of the healing infarct zone was significantly higher in the Gel@MSN/miR-21-5p treatment group than in the other groups. The CD31 and alpha-SMA staining intensities in the above-described groups were quantitatively analyzed (scale bars, 500 mm). * P < 0.05 and *** P < 0.01. Sham, n = 3; MI/saline, n = 5; MI/agomir, n = 5; MI/Gel@MSN/miR-NC, n = 6; and MI/Gel@MSN/miR-21-5p, n = 6. The data are shown as the means +- SD. Photo credit: Yan Li, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.

Product Details

16-0319-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Flow Cytometry (Flow)

0.25 µg/test

View 26 publications 26 publications

Functional Assay (FN)

Assay-Dependent

Control (Ctrl)

Assay-Dependent

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

WM-59 (WM59)

Conjugate

Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

RRID

AB_468934

Product Specific Information

Description: The WM59 monoclonal antibody reacts with human CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. This 130-140 kDa surface protein is expressed by endothelial cells and at low levels on leukocytes and platelets. It has been reported that CD38 binds to CD31. Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction.

Applications Reported: The WM-59 (WM59) antibody has been reported for use in flow cytometric analysis.

Applications Tested: The WM-59 (WM59) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Expression and role of interleukin-1β and associated biomarkers in deep vein thrombosis.

Experimental and therapeutic medicine

Pai RZ,Fang Q,Tian G,Zhu B,Ge X

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Flow Cytometry

Wed Dec 01 00:00:00 EST 2021

Simultaneously targeting cancer-associated fibroblasts and angiogenic vessel as a treatment for TNBC.

The Journal of experimental medicine

Sharma M,Turaga RC,Yuan Y,Satyanarayana G,Mishra F,Bian Z,Liu W,Sun L,Yang J,Liu ZR

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Immunocytochemistry

Mon Apr 05 00:00:00 EDT 2021

Simultaneously targeting cancer-associated fibroblasts and angiogenic vessel as a treatment for TNBC.

The Journal of experimental medicine

Sharma M,Turaga RC,Yuan Y,Satyanarayana G,Mishra F,Bian Z,Liu W,Sun L,Yang J,Liu ZR

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Immunocytochemistry

Mon Apr 05 00:00:00 EDT 2021

Injectable hydrogel with MSNs/microRNA-21-5p delivery enables both immunomodification and enhanced angiogenesis for myocardial infarction therapy in pigs.

Science advances

Li Y,Chen X,Jin R,Chen L,Dang M,Cao H,Dong Y,Cai B,Bai G,Gooding JJ,Liu S,Zou D,Zhang Z,Yang C

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Injectable hydrogel with MSNs/microRNA-21-5p delivery enables both immunomodification and enhanced angiogenesis for myocardial infarction therapy in pigs.

Science advances

Li Y,Chen X,Jin R,Chen L,Dang M,Cao H,Dong Y,Cai B,Bai G,Gooding JJ,Liu S,Zou D,Zhang Z,Yang C

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Increased infiltration and tolerised antigen-specific CD8⁺ T_EM cells in tumor but not peripheral blood have no impact on survival of HCMV⁺ glioblastoma patients.

Oncoimmunology

Bahador M,Gras Navarro A,Rahman MA,Dominguez-Valentin M,Sarowar S,Ulvestad E,Njølstad G,Lie SA,Kristoffersen EK,Bratland E,Chekenya M

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair.

Frontiers in cell and developmental biology

Sun L,Zhu W,Zhao P,Zhang J,Lu Y,Zhu Y,Zhao W,Liu Y,Chen Q,Zhang F

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Placental endovascular extravillous trophoblasts (enEVTs) educate maternal T-cell differentiation along the maternal-placental circulation.

Cell proliferation

Ma Y,Yang Q,Fan M,Zhang L,Gu Y,Jia W,Li Z,Wang F,Li YX,Wang J,Li R,Shao X,Wang YL

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Flow Cytometry

Fri May 01 00:00:00 EDT 2020

PF-127 hydrogel plus sodium ascorbyl phosphate improves Wharton's jelly mesenchymal stem cell-mediated skin wound healing in mice.

Stem cell research & therapy

Deng Q,Huang S,Wen J,Jiao Y,Su X,Shi G,Huang J

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Flow Cytometry

Fri Apr 03 00:00:00 EDT 2020

Matrine inhibits the development and progression of ovarian cancer by repressing cancer associated phosphorylation signaling pathways.

Cell death & disease

Zhang X,Hou G,Liu A,Xu H,Guan Y,Wu Y,Deng J,Cao X

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Immunohistochemistry

Thu Oct 10 00:00:00 EDT 2019

Mesenchymal stromal cells lower platelet activation and assist in platelet formation in vitro.

JCI insight

Mendelson A,Strat AN,Bao W,Rosston P,Fallon G,Ohrn S,Zhong H,Lobo C,An X,Yazdanbakhsh K

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Immunohistochemistry

Thu Aug 22 00:00:00 EDT 2019

CRISPR/Cas9‑mediated hypoxia inducible factor‑1α knockout enhances the antitumor effect of transarterial embolization in hepatocellular carcinoma.

Oncology reports

Liu Q,Fan D,Adah D,Wu Z,Liu R,Yan QT,Zhang Y,Du ZY,Wang D,Li Y,Bao SY,Liu LP

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Immunohistochemistry (Paraffin)

Thu Nov 01 00:00:00 EDT 2018

CRISPR/Cas9‑mediated hypoxia inducible factor‑1α knockout enhances the antitumor effect of transarterial embolization in hepatocellular carcinoma.

Oncology reports

Liu Q,Fan D,Adah D,Wu Z,Liu R,Yan QT,Zhang Y,Du ZY,Wang D,Li Y,Bao SY,Liu LP

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Immunohistochemistry (Paraffin)

Thu Nov 01 00:00:00 EDT 2018

Spatial and Single-Cell Transcriptional Profiling Identifies Functionally Distinct Human Dermal Fibroblast Subpopulations.

The Journal of investigative dermatology

Philippeos C,Telerman SB,Oulès B,Pisco AO,Shaw TJ,Elgueta R,Lombardi G,Driskell RR,Soldin M,Lynch MD,Watt FM

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Prostaglandin E₂ Is Required for BMP4-Induced Mesoderm Differentiation of Human Embryonic Stem Cells.

Stem cell reports

Zhang B,He L,Liu Y,Zhang J,Zeng Q,Wang S,Fan Z,Fang F,Chen L,Lv Y,Xi J,Yue W,Li Y,Pei X

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Flow Cytometry

Tue Mar 13 00:00:00 EDT 2018

Expansion of functional personalized cells with specific transgene combinations.

Nature communications

Lipps C,Klein F,Wahlicht T,Seiffert V,Butueva M,Zauers J,Truschel T,Luckner M,Köster M,MacLeod R,Pezoldt J,Hühn J,Yuan Q,Müller PP,Kempf H,Zweigerdt R,Dittrich-Breiholz O,Pufe T,Beckmann R,Drescher W,Riancho J,Sañudo C,Korff T,Opalka B,Rebmann V,Göthert JR,Alves PM,Ott M,Schucht R,Hauser H,Wirth D,May T

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Flow Cytometry

Thu Mar 08 00:00:00 EST 2018

Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.

Stem cell reports

Li Y,Brauer PM,Singh J,Xhiku S,Yoganathan K,Zúñiga-Pflücker JC,Anderson MK

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Flow Cytometry

Tue Sep 12 00:00:00 EDT 2017

ERK signaling is required for VEGF-A/VEGFR2-induced differentiation of porcine adipose-derived mesenchymal stem cells into endothelial cells.

Stem cell research & therapy

Almalki SG,Agrawal DK

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Flow Cytometry

Fri May 12 00:00:00 EDT 2017

MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells.

Stem cells translational medicine

Almalki SG,Llamas Valle Y,Agrawal DK

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2017

In Vitro Modeling of Blood-Brain Barrier with Human iPSC-Derived Endothelial Cells, Pericytes, Neurons, and Astrocytes via Notch Signaling.

Stem cell reports

Yamamizu K,Iwasaki M,Takakubo H,Sakamoto T,Ikuno T,Miyoshi M,Kondo T,Nakao Y,Nakagawa M,Inoue H,Yamashita JK

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Flow Cytometry

Tue Mar 14 00:00:00 EDT 2017

Endothelial and smooth muscle cells derived from human cardiac explants demonstrate angiogenic potential and suitable for design of cell-containing vascular grafts.

Journal of translational medicine

Zakharova IS,Zhiven' MK,Saaya SB,Shevchenko AI,Smirnova AM,Strunov A,Karpenko AA,Pokushalov EA,Ivanova LN,Makarevich PI,Parfyonova YV,Aboian E,Zakian SM

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Immunofluorescence

Fri Mar 03 00:00:00 EST 2017

TGF-β reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24- cancer cells.

eLife

Pal D,Pertot A,Shirole NH,Yao Z,Anaparthy N,Garvin T,Cox H,Chang K,Rollins F,Kendall J,Edwards L,Singh VA,Stone GC,Schatz MC,Hicks J,Hannon GJ,Sordella R

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Flow Cytometry

Mon Jan 16 00:00:00 EST 2017

Acidic pH reduces VEGF-mediated endothelial cell responses by downregulation of VEGFR-2; relevance for anti-angiogenic therapies.

Oncotarget

Faes S,Uldry E,Planche A,Santoro T,Pythoud C,Demartines N,Dormond O

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Immunofluorescence

Tue Dec 27 00:00:00 EST 2016

Oxidized macrophage migration inhibitory factor is a potential new tissue marker and drug target in cancer.

Oncotarget

Schinagl A,Thiele M,Douillard P,Völkel D,Kenner L,Kazemi Z,Freissmuth M,Scheiflinger F,Kerschbaumer RJ

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Immunofluorescence

Tue Nov 08 00:00:00 EST 2016

CXCL12/CXCR4 activation by cancer-associated fibroblasts promotes integrin β1 clustering and invasiveness in gastric cancer.

International journal of cancer

Izumi D,Ishimoto T,Miyake K,Sugihara H,Eto K,Sawayama H,Yasuda T,Kiyozumi Y,Kaida T,Kurashige J,Imamura Y,Hiyoshi Y,Iwatsuki M,Iwagami S,Baba Y,Sakamoto Y,Miyamoto Y,Yoshida N,Watanabe M,Takamori H,Araki N,Tan P,Baba H

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Induced Pluripotent Stem Cells to Model Human Fibrodysplasia Ossificans Progressiva.

Stem cell reports

Cai J,Orlova VV,Cai X,Eekhoff EMW,Zhang K,Pei D,Pan G,Mummery CL,Ten Dijke P

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Flow Cytometry

Tue Dec 08 00:00:00 EST 2015

Human esophageal myofibroblasts secrete proinflammatory cytokines in response to acid and Toll-like receptor 4 ligands.

American journal of physiology. Gastrointestinal and liver physiology

Gargus M,Niu C,Vallone JG,Binkley J,Rubin DC,Shaker A

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2015

Mesenchymal stromal cells form vascular tubes when placed in fibrin sealant and accelerate wound healing in vivo.

Biomaterials

Mendez JJ,Ghaedi M,Sivarapatna A,Dimitrievska S,Shao Z,Osuji CO,Steinbacher DM,Leffell DJ,Niklason LE

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Immunofluorescence

Sun Feb 01 00:00:00 EST 2015

Mesenchymal stromal cells form vascular tubes when placed in fibrin sealant and accelerate wound healing in vivo.

Biomaterials

Mendez JJ,Ghaedi M,Sivarapatna A,Dimitrievska S,Shao Z,Osuji CO,Steinbacher DM,Leffell DJ,Niklason LE

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Immunofluorescence

Sun Feb 01 00:00:00 EST 2015

Activation of the Wnt/planar cell polarity pathway is required for pericyte recruitment during pulmonary angiogenesis.

The American journal of pathology

Yuan K,Orcholski ME,Panaroni C,Shuffle EM,Huang NF,Jiang X,Tian W,Vladar EK,Wang L,Nicolls MR,Wu JY,de Jesus Perez VA

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2015

Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer.

Cell death & disease

Feng Y,Zhu M,Dangelmajer S,Lee YM,Wijesekera O,Castellanos CX,Denduluri A,Chaichana KL,Li Q,Zhang H,Levchenko A,Guerrero-Cazares H,Quiñones-Hinojosa A

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Flow Cytometry

Thu Dec 11 00:00:00 EST 2014

Autosomal dominant immune dysregulation syndrome in humans with CTLA4 mutations.

Nature medicine

Schubert D,Bode C,Kenefeck R,Hou TZ,Wing JB,Kennedy A,Bulashevska A,Petersen BS,Schäffer AA,Grüning BA,Unger S,Frede N,Baumann U,Witte T,Schmidt RE,Dueckers G,Niehues T,Seneviratne S,Kanariou M,Speckmann C,Ehl S,Rensing-Ehl A,Warnatz K,Rakhmanov M,Thimme R,Hasselblatt P,Emmerich F,Cathomen T,Backofen R,Fisch P,Seidl M,May A,Schmitt-Graeff A,Ikemizu S,Salzer U,Franke A,Sakaguchi S,Walker LSK,Sansom DM,Grimbacher B

16-0319 was used in Flow cytometry/Cell sorting to demonstrate the consequences for mice with mutations in CTLA4, an essential negative regulator of immune responses.

Mon Dec 01 00:00:00 EST 2014

Vascular progenitors from cord blood-derived induced pluripotent stem cells possess augmented capacity for regenerating ischemic retinal vasculature.

Circulation

Park TS,Bhutto I,Zimmerlin L,Huo JS,Nagaria P,Miller D,Rufaihah AJ,Talbot C,Aguilar J,Grebe R,Merges C,Reijo-Pera R,Feldman RA,Rassool F,Cooke J,Lutty G,Zambidis ET

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Flow Cytometry

Tue Jan 21 00:00:00 EST 2014

Cells expressing the C/EBPbeta isoform, LIP, engulf their neighbors.

PloS one

Abreu M,Sealy L

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Flow Cytometry

Thu Apr 11 00:00:00 EDT 2013

Efficient and simultaneous generation of hematopoietic and vascular progenitors from human induced pluripotent stem cells.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Park TS,Zimmerlin L,Zambidis ET

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2013

Efficient direct reprogramming of mature amniotic cells into endothelial cells by ETS factors and TGFβ suppression.

Cell

Ginsberg M,James D,Ding BS,Nolan D,Geng F,Butler JM,Schachterle W,Pulijaal VR,Mathew S,Chasen ST,Xiang J,Rosenwaks Z,Shido K,Elemento O,Rabbany SY,Rafii S

Published figure using CD31 (PECAM-1) monoclonal antibody (Product # 16-0319-82) in Flow Cytometry

Fri Oct 26 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: CD31; CD31 antigen; EndoCAM; GPIIA'; PECA1; PECAM-1; Platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule 1

Gene Aliases: CD31; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1; PECAM1

UniProt ID: (Human) Q9NPB7

Entrez Gene ID: (Human) 5175

Molecular Function: immunoglobulin receptor superfamily

Function(s)

platelet degranulation phagocytosis cell adhesion signal transduction cell recognition extracellular matrix organization leukocyte migration diapedesis glomerular endothelium development

Process(es)

protein binding

CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), Functional Grade, eBioscience™

Product Details

16-0319-82

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Functional Assay (FN)

Control (Ctrl)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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