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|Tested species reactivity||Mouse|
|Host / Isotype||Rat / IgG2a|
|Immunogen||T-end.1, a pMT transformed mouse endothelial cell line|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:25-1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Immunoprecipitation (IP)||2 -10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CD34 antigen; cluster designation 34; hematopoietic progenitor cell antigen CD34; Mucosialin; RP11-328D5.2