|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Human CD3 delta|
|Conjugate||APC-Alexa Fluor® 750|
|Storage buffer||PBS with BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 2 publications below|
The allophycocyanin (APC)-Alexa Fluor® 750 dye tandem conjugate allows for multicolor measurements using excitation by lasers that emit from 633 to 650 nm.
The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined.
Analyte Specific Reagent
High numbers of differentiated effector CD4 T cells are found in patients with cancer and correlate with clinical response after neoadjuvant therapy of breast cancer.
MHCD0327 was used in flow cytometry to examine human differentiated effector CD4(+) T cells that are defined by low levels of IL-2 and IL-7 receptors.
|Péguillet I,Milder M,Louis D,Vincent-Salomon A,Dorval T,Piperno-Neumann S,Scholl SM,Lantz O||Cancer research (74:2204)||2014|
Human neonatal naive CD4+ T cells have enhanced activation-dependent signaling regulated by the microRNA miR-181a.
MHCD0327 was used in flow cytometry to test if impaired human neonatal CD4(+) T cell immunity is due to reduced signaling by naive CD4(+) T cells following engagement of the αβ-TCR/CD3 complex and CD28.
|Palin AC,Ramachandran V,Acharya S,Lewis DB||Journal of immunology (Baltimore, Md. : 1950) (190:2682)||2013|