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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic 13-mer peptide corresponding to internal region of the epsilon chain of human CD3 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Ready-to-use|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with equine, feline, non-human primate, and canine based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is tonsil tissue.
CD3 is present on 70% of normal peripheral blood lymphocytes and 60% of thymocytes. This antigen is found on the majority of human T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CD3-epsilon; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); epsilon polypeptide TiT3 complex; epsilon subunit of T3; FLJ18683; T-cell antigen receptor complex; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T3E; TCRE
CD3E; IMD18; T3E; TCRE