Immunofluorescence analysis of CD3e + CD3d + CD3g was done on 70% confluent log phase Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD3e + CD3d + CD3g (F7.2.38) Mouse Monoclonal Antibody (MA512577) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Purified CD3 epsilon gamma delta/CD3omega|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:20|
|Western Blot (WB)||1:250-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
MA5-12577 targets CD3E in IHC (P) applications and shows reactivity with Human samples.
The MA5-12577 immunogen is purified CD3 epsilon gamma delta/CD3omega.
CD3 consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta). The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). It is involved in signal transduction to the T cell interior following antigen recognition. CD3 antigen is a highly specific marker for T cells and is present in majority of T cell neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Increase in tumour-infiltrating lymphocytes with regulatory T cell immunophenotypes and reduced zeta-chain expression in nasopharyngeal carcinoma patients.
MA5-12577 was used in immunohistochemistry to investigate the tumour-infiltrating lymphocytes in nasopharyngeal carcinoma patients
|Yip WK,Abdullah MA,Yusoff SM,Seow HF||Clinical and experimental immunology (155:412)||2009|