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Immunofluorescent analysis of CD4 (green) on the surface of D10.G4.1 mouse T-lymphocytes. Formalin fixed cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a CD4 monoclonal antibody (Product # MA1-146) at a dilution of 1:20 for 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rat IgG secondary antibody (Product # SA5-10018). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Mouse|
|Host / Isotype||Rat / IgG2b, kappa|
|Immunogen||Mouse CTL clone V4|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Flow cytometry analysis of MA1-146 shows positive staining of mouse T-lymphocytes. The antibody also detects recombinant mouse CD4 at ~55 kDa.
This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CD4 antigen p55; CD4 receptor; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)
Cd4; L3T4; Ly-4