Immunofluorescence analysis of CD44v6 was performed using 90% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD44v6 (MA54) Mouse Monoclonal Antibody (33-6700) at 2 µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conj µgate (A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization in the membrane. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||epitope encoded by exon v6 on the variant portion of human CD44|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||2 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD44 cell surface antigen is a 100kDa glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Co-expression of CD133, CD44v6 and human tissue factor is associated with metastasis and poor prognosis in pancreatic carcinoma.
33-6700 was used in immunohistochemistry - paraffin section to determine whether co-expression of CD133, CD44v6, and human tissue factor are associated with metastasis and overall prognosis in pancreatic carcinoma
|Chen K,Li Z,Jiang P,Zhang X,Zhang Y,Jiang Y,He Y,Li X||Oncology reports (32:755)||2014|
|Human||Not Cited||Leuprorelin acetate affects adhesion molecule expression in human prostate cancer cells.||Angelucci C,Lama G,Iacopino F,Sica G||International journal of oncology (38:1501)||2011|
||De novo expression of CD44 variants in sporadic and hereditary gastric cancer.||da Cunha CB,Oliveira C,Wen X,Gomes B,Sousa S,Suriano G,Grellier M,Huntsman DG,Carneiro F,Granja PL,Seruca R||Laboratory investigation; a journal of technical methods and pathology (90:1604)||2010|
|Human||1:100||De novo expression of CD44 variants in sporadic and hereditary gastric cancer.||da Cunha CB,Oliveira C,Wen X,Gomes B,Sousa S,Suriano G,Grellier M,Huntsman DG,Carneiro F,Granja PL,Seruca R||Laboratory investigation; a journal of technical methods and pathology (90:1604)||2010|