Invitrogen

CD61 (Integrin beta 3) Monoclonal Antibody (2C9.G3), eBioscience™

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

CD61 (Integrin beta 3) Antibody in Flow Cytometry (Flow) — Staining of C57Bl/6 bone marrow cells with 0.25 µg Armenian Hamster IgG Isotype Control Purified (Product # 14-4888-81) (open histogram) or 0.25 µg of Anti-Mouse/Rat CD61 (Integrin beta 3) Purified (filled histogram) followed byAnti-Armenian Hamster IgG Biotin (Product # 13-4113-85) and Streptavidin PE (Product # 12-4317-87). Cells in the large scatter population were used for analysis.

CD61 (Integrin beta 3) Antibody in Immunocytochemistry (ICC/IF) — Figure 2. The FN synergy site is dispensable for FN fibrillogenesis, cell adhesion and spreading. ( A ) Fn1 -Knock-Out ( Fn1 -KO) fibroblasts grown in 1% plasma derived from either Fn1 +/+ or Fn1 syn/syn mice, fixed at the indicated times and stained for FN (green), F-actin stain (with Phalloidin; red) and nuclei (with DAPI; blue). Scale bar, 10 mum. ( B ) Fn1 -KO cells seeded on pFN wt or pFN syn , fixed at the indicated times and stained for F-actin (red), paxillin (white) and total beta1 integrin (green). Scale bar, 20 mum. ( C-E ) Cell size ( C ), number of FAs per cell ( D ) and percentage coverage by FAs (paxillin-positive) ( E ) were quantified (n = 25 cells assessed from three independent experiments; mean +- sem). Statistical significances were calculated using the Student t -test; **p < 0.01 and ***p < 0.001. DOI: http://dx.doi.org/ Figure 2--figure supplement 1. Integrin surface levels and plasma FN purification and glass coating. ( A ) Integrin levels on the surface of Fn1 -KO fibroblasts analyzed by flow cytometry (graphs show representative results of 3 independent experiments). ( B ) Coomasie blue staining of purified pFN wt and pFN syn after PAGE. The second lane shows the starting flow-through, and the 5-11 th the fractions with FN. ( C ) Western-blot of the purified pFN wt and pFN syn . ( D ) Western-blot to detect fibrinogen in the purified mouse pFN. The fibrinogen content was below the 4% of protein. ( E ) ELISA of glass-coverslips coated with different p

CD61 (Integrin beta 3) Antibody in Immunohistochemistry (IHC) — Figure 1 beta3-integrin expression is upregulated in human dilated and ischaemic cardiomyopathy. (A) Western blotting analysis of beta3 integrin in protein lysates from human non-failing heart, non-failing heart with hypertrophy, and dilated and ischaemic myocardium. GAPDH was used as a loading control. Bar charts show mean densitometric readings +- SEM. N , 5 or 6 individual patient samples. (B) Immunofluorescence reveals beta3-integrin expression in sections of human non-failing heart with an apparent increase in dilated and ischaemic hearts, but less in non-failing heart with hypertrophy. The non-specific primary antibody control showed minimal background level staining. N , four individual patient samples per group. Scale bar = 50 mum. Student's t -test, * p < 0.05. PATH-5340-FIG-0001-c

CD61 (Integrin beta 3) Antibody in Western Blot (WB) — Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 14-0611-85) in Western Blot

CD61 (Integrin beta 3) Antibody in Neutralization (Neu) — Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 14-0611-85) in Neutralization

Product Details

14-0611-85

Applications	Tested Dilution	Publications
Immunohistochemistry (Frozen) (IHC (F))	Assay-Dependent	-
Flow Cytometry (Flow)	0.5 µg/test	View 9 publications 9 publications
Neutralization (Neu)	Assay-Dependent	View 2 publications 2 publications
Functional Assay (FN)	Assay-Dependent	-
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunocytochemistry (ICC/IF)	-	View 3 publications 3 publications
Western Blot (WB)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Mouse, Rat
Published species	Mouse
Host/Isotype	Armenian hamster / IgG
Class	Monoclonal
Type	Antibody
Clone	2C9.G3
Conjugate	Unconjugated APC Biotin FITC Functional Grade NovaFluor Blue 610-70S NovaFluor Blue 660-120S NovaFluor Blue 690 NovaFluor Red 725 NovaFluor Yellow 570 NovaFluor Yellow 660 NovaFluor Yellow 730 NovaFluor Yellow 755 PE
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_467312

Product Specific Information

Description: The 2C9.G3 (HMb3-1) monoclonal antibody reacts with mouse and rat CD61, also known as the integrin beta3. CD61 is expressed by activated T cells, granulocytes, and platelet. CD61 associates non-covalently with two integrin alpha subunits; alphaV (CD51) to form Vitronectin Receptor and alphaIib (CD41) to form gpIIb/IIIa. These heterodimeric complexes are responsible for adhesion to extracellular matrix components including fibrinogen, fibronectin, fibronectin, vitronectin, thrombospondin and von Willebrand factor.

Applications Reported: The 2C9.G3 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. 2C9.G3 has also been reported in blocking of ligand binding and some adhesive processes. (Please use Functional Grade purified 2C9.G3, cat. 16-0611, in functional assays.).

Applications Tested: The 2C9.G3 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD61 (GPIIIa, ITGB3) is a glycoprotein found on megakaryocytes, platelets and their precursors. CD61 antigen plays a role in platelet aggregation and also as a receptor for fibrinogen, fibronectin, von Willebrand factor and vitronectrin. CD61 is a 105 kDa glycoprotein that associates with either the alpha 11b integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51. CD61 is a receptor for fibrinogen, fibronectin, Von Willebrand Factor, vitronectin and thrombospondin. The CD61 protein product is composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. CD61 is found along with the alpha IIb chain in platelets. Like other integrins, CD61 is known to participate in cell adhesion as well as cell-surface mediated signaling. Diseases associated with CD61 dysfunction include Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Repurposing an anti-cancer agent for the treatment of hypertrophic heart disease.

The Journal of pathology

Dukinfield M,Maniati E,Reynolds LE,Aubdool A,Baliga RS,D'Amico G,Maiques O,Wang J,Bedi KC,Margulies KB,Sanz-Moreno V,Hobbs A,Hodivala-Dilke K

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 14-0611-85) in Immunohistochemistry

Sun Dec 01 00:00:00 EST 2019

Hoxa9 and Meis1 Cooperatively Induce Addiction to Syk Signaling by Suppressing miR-146a in Acute Myeloid Leukemia.

Cancer cell

Mohr S,Doebele C,Comoglio F,Berg T,Beck J,Bohnenberger H,Alexe G,Corso J,Ströbel P,Wachter A,Beissbarth T,Schnütgen F,Cremer A,Haetscher N,Göllner S,Rouhi A,Palmqvist L,Rieger MA,Schroeder T,Bönig H,Müller-Tidow C,Kuchenbauer F,Schütz E,Green AR,Urlaub H,Stegmaier K,Humphries RK,Serve H,Oellerich T

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 14-0611-85) in Flow Cytometry

Mon Apr 10 00:00:00 EDT 2017

The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes.

eLife

Benito-Jardón M,Klapproth S,Gimeno-LLuch I,Petzold T,Bharadwaj M,Müller DJ,Zuchtriegel G,Reichel CA,Costell M

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 14-0611-85) in Flow Cytometry

Mon Jan 16 00:00:00 EST 2017

The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes.

eLife

Benito-Jardón M,Klapproth S,Gimeno-LLuch I,Petzold T,Bharadwaj M,Müller DJ,Zuchtriegel G,Reichel CA,Costell M

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 14-0611-85) in Flow Cytometry

Mon Jan 16 00:00:00 EST 2017

WISP1-αvβ3 integrin signaling positively regulates TLR-triggered inflammation response in sepsis induced lung injury.

Scientific reports

Chen Z,Ding X,Jin S,Pitt B,Zhang L,Billiar T,Li Q

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 14-0611-85) in Neutralization

Tue Jun 28 00:00:00 EDT 2016

WISP1-αvβ3 integrin signaling positively regulates TLR-triggered inflammation response in sepsis induced lung injury.

Scientific reports

Chen Z,Ding X,Jin S,Pitt B,Zhang L,Billiar T,Li Q

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 14-0611-85) in Neutralization

Tue Jun 28 00:00:00 EDT 2016

Dicer1-mediated miRNA processing shapes the mRNA profile and function of murine platelets.

Blood

Rowley JW,Chappaz S,Corduan A,Chong MM,Campbell R,Khoury A,Manne BK,Wurtzel JG,Michael JV,Goldfinger LE,Mumaw MM,Nieman MT,Kile BT,Provost P,Weyrich AS

14-0611 was used in Flow cytometry/Cell sorting to study the role of Dicer1 in the regulation of integrin expression by platelets.

Thu Apr 07 00:00:00 EDT 2016

H2S protects against fatal myelosuppression by promoting the generation of megakaryocytes/platelets.

Journal of hematology & oncology

Liu HD,Zhang AJ,Xu JJ,Chen Y,Zhu YC

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 14-0611-85) in Immunofluorescence

Wed Feb 24 00:00:00 EST 2016

The matricellular protein CCN1 mediates neutrophil efferocytosis in cutaneous wound healing.

Nature communications

Jun JI,Kim KH,Lau LF

14-0611 was used in Blocking experiments to establish the matricellular protein, CCN1, as a critical opsonin in skin injury.

Tue Jun 16 00:00:00 EDT 2015

Evidence for a multipotent mammary progenitor with pregnancy-specific activity.

Breast cancer research : BCR

Kaanta AS,Virtanen C,Selfors LM,Brugge JS,Neel BG

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 14-0611-85) in Immunofluorescence

Mon Dec 08 00:00:00 EST 2014

β1 integrins with individually disrupted cytoplasmic NPxY motifs are embryonic lethal but partially active in the epidermis.

The Journal of investigative dermatology

Meves A,Stremmel C,Böttcher RT,Fässler R

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 14-0611-85) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2013

Integrin-modulating therapy prevents fibrosis and autoimmunity in mouse models of scleroderma.

Nature

Gerber EE,Gallo EM,Fontana SC,Davis EC,Wigley FM,Huso DL,Dietz HC

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 14-0611-85) in Flow Cytometry

Thu Nov 07 00:00:00 EST 2013

Integrin-mediated internalization of Staphylococcus aureus does not require vinculin.

BMC cell biology

Borisova M,Shi Y,Buntru A,Wörner S,Ziegler WH,Hauck CR

14-0611 was used in Flow cytometry/Cell sorting to suggest that vinculin is insignificant for the integrin-mediated uptake of Staphylococcus aureus.

Mon Jan 07 00:00:00 EST 2013

The interplay between surfaces and soluble factors define the immunologic and angiogenic properties of myeloid dendritic cells.

BMC immunology

Sprague L,Muccioli M,Pate M,Meles E,McGinty J,Nandigam H,Venkatesh AK,Gu MY,Mansfield K,Rutowski A,Omosebi O,Courreges MC,Benencia F

14-0611 was used in Flow cytometry/Cell sorting to study the effects of different surfaces and soluble factors on the biology of dendritic cells.

Mon Jun 06 00:00:00 EDT 2011

Differentiation generates paracrine cell pairs that maintain basaloid mouse mammary tumors: proof of concept.

PloS one

Kim S,Goel S,Alexander CM

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 14-0611-85) in Flow Cytometry

Tue Apr 26 00:00:00 EDT 2011

Plasminogen activator inhibitor-1 regulates integrin alphavbeta3 expression and autocrine transforming growth factor beta signaling.

The Journal of biological chemistry

Pedroja BS,Kang LE,Imas AO,Carmeliet P,Bernstein AM

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 14-0611-85) in Immunofluorescence

Fri Jul 31 00:00:00 EDT 2009

Plasminogen activator inhibitor-1 regulates integrin alphavbeta3 expression and autocrine transforming growth factor beta signaling.

The Journal of biological chemistry

Pedroja BS,Kang LE,Imas AO,Carmeliet P,Bernstein AM

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 14-0611-85) in Immunofluorescence

Fri Jul 31 00:00:00 EDT 2009

Constitutively active aryl hydrocarbon receptor expressed specifically in T-lineage cells causes thymus involution and suppresses the immunization-induced increase in splenocytes.

Journal of immunology (Baltimore, Md. : 1950)

Nohara K,Pan X,Tsukumo S,Hida A,Ito T,Nagai H,Inouye K,Motohashi H,Yamamoto M,Fujii-Kuriyama Y,Tohyama C

Tue Mar 01 00:00:00 EST 2005

Bioinformatics

Protein Aliases: CD61; GPIIIa; integrin beta 3 (Cd61); Integrin beta-3; platelet gpIIIa; Platelet membrane glycoprotein IIIa

Gene Aliases: CD61; GP3A; INGRB3; Itgb3

UniProt ID: (Rat) Q8R2H2, (Mouse) O54890

Entrez Gene ID: (Rat) 29302, (Mouse) 16416

Molecular Function: integrin

Function(s)

Process(es)

fibronectin binding protease binding protein disulfide isomerase activity integrin binding protein binding peptide binding identical protein binding vascular endothelial growth factor receptor 2 binding cell adhesion molecule binding extracellular matrix binding fibrinogen binding receptor activity

CD61 (Integrin beta 3) Monoclonal Antibody (2C9.G3), eBioscience™

Product Details

14-0611-85

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (FN)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!