Invitrogen

CD61 (Integrin beta 3) Monoclonal Antibody (2C9.G3), eBioscience™

Product Image Gallery

CD61 (Integrin beta 3) Antibody — Figure 3 Syk Phosphorylation Is Partly Dependent on Integrin Beta 3 (A) Co-immunoprecipitation of Fcer1g and Syk from H and H/M cells. (B) Fcer1g , Itgb3 , and Itgav expression estimated by normalized RNA-seq counts. (C) Itgb3 and Itgav cell surface expression in H and H/M cells measured by flow cytometry. Unstained cells were used as controls. (D) Itgb3 cell surface expression in H/M cells transduced with either a lentiviral non-specific (nsp) control CRISPR or a CRISPR targeting Itgb3 (DeltaItgb3). (E) Corresponding (p)Syk expression determined by immunoblotting. Actin was used as loading control for relative protein quantification.

CD61 (Integrin beta 3) Antibody in Flow Cytometry (Flow) — Staining of C57Bl/6 bone marrow cells with 0.25 µg Armenian Hamster IgG Isotype Control Purified (Product # 14-4888-81) (open histogram) or 0.25 µg of Anti-Mouse/Rat CD61 (Integrin beta 3) Purified (filled histogram) followed byAnti-Armenian Hamster IgG Biotin (Product # 13-4113-85) and Streptavidin PE (Product # 12-4317-87). Cells in the large scatter population were used for analysis.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

0.5 µg/test

Functional Assay (FN)

Assay-Dependent

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Neutralization (Neu)

Assay-Dependent

Published Applications

Flow Cytometry (Flow)

See 8 publications below

Immunofluorescence (IF)

See 4 publications below

Western Blot (WB)

See 2 publications below

Neutralization (Neu)

See 1 publication below

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Mouse

Host / Isotype

Armenian hamster / IgG

Class

Monoclonal

Type

Antibody

Clone

2C9.G3

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_467312

Product Specific Information

Description: The 2C9.G3 (HMb3-1) monoclonal antibody reacts with mouse and rat CD61, also known as the integrin beta3. CD61 is expressed by activated T cells, granulocytes, and platelet. CD61 associates non-covalently with two integrin alpha subunits; alphaV (CD51) to form Vitronectin Receptor and alphaIib (CD41) to form gpIIb/IIIa. These heterodimeric complexes are responsible for adhesion to extracellular matrix components including fibrinogen, fibronectin, fibronectin, vitronectin, thrombospondin and von Willebrand factor.

Applications Reported: The 2C9.G3 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. 2C9.G3 has also been reported in blocking of ligand binding and some adhesive processes. (Please use Functional Grade purified 2C9.G3, cat. 16-0611, in functional assays.).

Applications Tested: The 2C9.G3 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: BDPLT2; CD61; GP3A; GPIIIa; integrin beta 3 (Cd61); Integrin beta-3; ITGB3; platelet glycoprotein IIIa; platelet gpIIIa; Platelet membrane glycoprotein IIIa

Gene Aliases: CD61; GP3A; INGRB3; Itgb3

UniProt ID: (Mouse) O54890

Entrez Gene ID: (Rat) 29302, (Mouse) 16416

Molecular Function: cell adhesion molecule extracellular matrix glycoprotein extracellular matrix protein receptor