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      Invitrogen

      CD61 (Integrin beta 3) Monoclonal Antibody (2C9.G3), eBioscience™

      Published Figures 14 Published Figures
      References Icon 15 References
      View (34) other CD61 (Integrin beta 3) antibodies
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      Catalog # 14-0611-85

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      Product Details

      TESTED APPLICATIONS DILUTION
      Flow Cytometry (Flow) 0.5 µg/test
      Functional Assay (FN) Assay-Dependent
      Immunohistochemistry (Frozen) (IHC (F)) Assay-Dependent
      Neutralization (Neu) Assay-Dependent
      PUBLISHED APPLICATIONS
      Flow Cytometry (Flow) See 7 publications below
      Immunofluorescence (IF) See 5 publications below
      Western Blot (WB) See 2 publications below
      Neutralization (Neu) See 1 publications below
      Species reactivity Mouse, Rat
      Published species Mouse
      Host / Isotype Armenian hamster / IgG
      Class Monoclonal
      Type Antibody
      Clone 2C9.G3
      Conjugate Unconjugated
      Form Liquid
      Concentration 0.5 mg/mL
      Purification Affinity chromatography
      Storage buffer PBS, pH 7.2
      Contains 0.09% sodium azide
      Storage conditions 4° C
      RRID AB_467312

      Product Specific Information

      Description: The 2C9.G3 (HMb3-1) monoclonal antibody reacts with mouse and rat CD61, also known as the integrin beta3. CD61 is expressed by activated T cells, granulocytes, and platelet. CD61 associates non-covalently with two integrin alpha subunits; alphaV (CD51) to form Vitronectin Receptor and alphaIib (CD41) to form gpIIb/IIIa. These heterodimeric complexes are responsible for adhesion to extracellular matrix components including fibrinogen, fibronectin, fibronectin, vitronectin, thrombospondin and von Willebrand factor.

      Applications Reported: The 2C9.G3 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. 2C9.G3 has also been reported in blocking of ligand binding and some adhesive processes. (Please use Functional Grade purified 2C9.G3, cat. 16-0611, in functional assays.).

      Applications Tested: The 2C9.G3 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

      Purity: Greater than 90%, as determined by SDS-PAGE.

      Aggregation: Less than 10%, as determined by HPLC.

      Filtration: 0.2 µm post-manufacturing filtered.

      Background/Target Information

      The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.

      For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

      References (15)

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      Bioinformatics

      Protein Aliases: BDPLT2; CD61; GP3A; GPIIIa; integrin beta 3 (Cd61); Integrin beta-3; ITGB3; platelet glycoprotein IIIa; platelet gpIIIa; Platelet membrane glycoprotein IIIa

      Gene Aliases: CD61; GP3A; INGRB3; Itgb3

      UniProt ID: (Mouse) O54890

      Entrez Gene ID: (Rat) 29302, (Mouse) 16416

      Molecular Function: cell adhesion molecule extracellular matrix glycoprotein extracellular matrix protein receptor

      Function(s) Blue Arrow Icon
      positive regulation of endothelial cell proliferation positive regulation of leukocyte migration protein folding cellular calcium ion homeostasis cell-substrate junction assembly cell adhesion cell-matrix adhesion integrin-mediated signaling pathway embryo implantation regulation of G-protein coupled receptor protein signaling pathway response to radiation positive regulation of endothelial cell migration negative regulation of macrophage derived foam cell differentiation positive regulation of fibroblast migration negative regulation of lipid storage response to activity smooth muscle cell migration positive regulation of smooth muscle cell migration cell growth cell migration platelet activation regulation of cell migration positive regulation of cell migration cell-substrate adhesion activation of protein kinase activity negative regulation of lipid transport positive regulation of cell adhesion mediated by integrin positive regulation of osteoblast proliferation heterotypic cell-cell adhesion substrate adhesion-dependent cell spreading cellular response to drug response to platelet-derived growth factor apolipoprotein A-I-mediated signaling pathway positive regulation of MAPK cascade negative regulation of low-density lipoprotein particle receptor biosynthetic process positive regulation of angiogenesis positive regulation of bone resorption viral entry into host cell mesodermal cell differentiation positive regulation of smooth muscle cell proliferation positive regulation of peptidyl-tyrosine phosphorylation negative regulation of lipoprotein metabolic process negative chemotaxis regulation of release of sequestered calcium ion into cytosol negative regulation of cell death positive regulation of ERK1 and ERK2 cascade platelet aggregation cellular response to mechanical stimulus positive regulation of glomerular mesangial cell proliferation positive regulation of adenylate cyclase-inhibiting opioid receptor signaling pathway cellular response to insulin-like growth factor stimulus positive regulation of osteoclast differentiation cell projection morphogenesis angiogenesis involved in wound healing
      Process(es) Blue Arrow Icon
      fibronectin binding
       protease binding
       protein disulfide isomerase activity
       integrin binding
       protein binding
       peptide binding
       identical protein binding
       vascular endothelial growth factor receptor 2 binding
       cell adhesion molecule binding
       extracellular matrix binding
       fibrinogen binding
       receptor activity

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