|Flow Cytometry (Flow)||0.5 µg/test|
|Functional Assay (FN)||Assay-Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay-Dependent|
|Flow Cytometry (Flow)||See 7 publications below|
|Immunofluorescence (IF)||See 5 publications below|
|Western Blot (WB)||See 2 publications below|
|Neutralization (Neu)||See 1 publications below|
|Species reactivity||Mouse, Rat|
|Host / Isotype||Armenian hamster / IgG|
|Storage buffer||PBS, pH 7.2|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
Description: The 2C9.G3 (HMb3-1) monoclonal antibody reacts with mouse and rat CD61, also known as the integrin beta3. CD61 is expressed by activated T cells, granulocytes, and platelet. CD61 associates non-covalently with two integrin alpha subunits; alphaV (CD51) to form Vitronectin Receptor and alphaIib (CD41) to form gpIIb/IIIa. These heterodimeric complexes are responsible for adhesion to extracellular matrix components including fibrinogen, fibronectin, fibronectin, vitronectin, thrombospondin and von Willebrand factor.
Applications Reported: The 2C9.G3 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. 2C9.G3 has also been reported in blocking of ligand binding and some adhesive processes. (Please use Functional Grade purified 2C9.G3, cat. 16-0611, in functional assays.).
Applications Tested: The 2C9.G3 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: BDPLT2; CD61; GP3A; GPIIIa; integrin beta 3 (Cd61); Integrin beta-3; ITGB3; platelet glycoprotein IIIa; platelet gpIIIa; Platelet membrane glycoprotein IIIa
Gene Aliases: CD61; GP3A; INGRB3; Itgb3
UniProt ID: (Mouse) O54890
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