Immunofluorescent analysis of CD8 (green) on the surface of TK-1 mouse T-cell lymphoma cells. Formalin fixed cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a CD8 monoclonal antibody (Product # MA1-145) at a dilution of 1:20 for 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rat IgG secondary antibody (Product # SA5-10018). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Mouse|
|Host / Isotype||Rat / IgG2b, kappa|
|Immunogen||Mouse CTL clone L3|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Flow cytometry analysis of MA1-145 shows positive staining of CD8a+ mouse TK-1 lymphocytes. Antibody not recommended for Western blot.
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain isoforms. Two alternative transcripts encoding distinct isoforms, one membrane associated and one secreted, have been identified [Gene Wiki: CD8a].
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